Heme and Micro math

Question 1

What are the 2 loop sizes mainly used for planting microbiology specimens?

Answer 1

.01mL (or 10uL) and .001mL (or 1uL)

Question 2

If I use a 1uL loop to streak out my urine culture plates, then every colony that forms would represent ______CFU/mL

Answer 2

1000

Question 3

If I use a 10uL loop to streak out my urine culture plates, then every colony that forms would represent ______ CFU/mL.

Answer 3

100

Question 4

Micro serial dilutions look at the effect of _________ over range of dilutions

Answer 4

antimicrobial

Question 5

________: one decimal point is standard. (Heme math)

Answer 5

Indices

Question 6

The volume of RBCs in compared to total volume of blood.

Answer 6

HCT (Hematocrit)

Question 7

The total concentration of Hgb in the blood

Answer 7

HGB (Hemoglobin)

Question 8

The size of a red cell.

Answer 8

MCV (Mean Corpuscular Volume)

Question 9

How much Hgb in one RBC.

Answer 9

MCH (Mean Corpuscular Hgb)

Question 10

How MCV and MCH factor together.

Answer 10

MCHC (Mean Corpuscular Hgb Concentration)

Question 11

What is the equation for MCV?

Answer 11

MCV (fl) = HCT (%) x 10 then divided by RBC count (millions per uL)

Question 12

What is the equation for MCH?

Answer 12

MCH (picogram) = HGB (g/dL) x 10 then divided by RBC count (millions per uL)

Question 13

What is the equation for MCHC?

Answer 13

MCHC (%) = HGB (g/dL) x 100 then divided by HCT (%)

Question 14

What is the equation from the hemacytometer?

Answer 14

cells/mm^3 = (cells counted) divided by (number of squares) then x volume correction then x dilution factor

Question 15

What is the volume correction for WBC squares?

Answer 15

10

Question 16

What is the volume correction for RBC squares?

Answer 16

250