Hemacytometer Flashcards
WBC Counts
Counted in 4 corner squares
RBC Counts
Counted in center and using the corners and dead center of grid
Platelet Counts
Center square
Low RBC Counts
Count more squares (less than 25 cells/square)
Use a lower dilution and recharge Hemacytometer
High WBC Counts
Count less squares
Use a higher dilution and recharge Hemacytometer
Area of counting grid
9 mm2
Volume of counting grid
0.9 cumm
WBC Calculation
(WBC x 20 X 10)/4
20 = dilution
10 = surface of hemacytometer
Fluid drying on hemacytometer
Falsely increase
Overfilling hemacytometer
Falsely increase
Not allowing WBC dilution to sit
Falsely increase
Inadequate charging time
Falsely decrease
RBC Calculation
(RBCs x 200 x 10)/(1/5)
200 - dilution
10 - hemacytometer volume
Platelet Calculation
(Platelets x 100 x 10)
100 - dilution
10 - hemacytometer volume
