Blood Smears and Staining

Question 1

Smear is too short

Answer 1

Use larger drop of blood, decrease angle of spreader slide

Question 2

Uneven Trailers

Answer 2

Spread blood more quickly and use even pressure

Question 3

Low Hematocrit Adjustment

Answer 3

Increase angle of spreader slide, spread blood more quickly

Question 4

Slide Stained Too Blue

Answer 4

Increase Buffer, decrease stain time, make buffer more acidic

Question 5

Slide Stained Too Pink

Answer 5

Increase stain time, make buffer less acidic

Question 6

Slide Stained Too Faint

Answer 6

Increase Buffer

Question 7

Slide Covered with Precipitated Stain

Answer 7

Clean platen, dip slide in methanol

Question 8

Methanol

Answer 8

Fixes smear

Question 9

Methylene Blue

Answer 9

Stains acidic components

Question 10

Eosin

Answer 10

Stains basic components

Question 11

Buffer

Answer 11

Staining occurs when buffer of proper pH is added and releases ions

Question 12

Increase angle

Answer 12

Shorter, thicker smear

Question 13

Decrease angle

Answer 13

Longer, thinner smear

Question 14

Smearing blood faster

Answer 14

Shorter, thicker smear

Question 15

Smearing blood slower

Answer 15

Longer, thinner smear

Question 16

Using large drop

Answer 16

Thicker smear

Question 17

Using smaller drop

Answer 17

Thinner smear