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AARALIN FOR PRACTICAL CC WEEK 1 > Hema Lab Hb And Veni > Flashcards

Hema Lab Hb And Veni Flashcards

1
Q

is the red iron-bearing protein
contained within the erythrocytes in
normal blood.

A

Hb (hemoglobin)

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2
Q

It is the carrier of oxygen from the
lungs to the tissue where it readily
releases this oxygen to the tissues and
then returns to the lungs to combine
with more oxygen.

A

Hemoglobin

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3
Q

represents the colored
component of blood

A

Heme or hemoglobin

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4
Q

It is a complex protein

A

Globin

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5
Q

The molecular weight of one Hb
molecule is

A

64,458 daltons

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6
Q

Hemoglobin results are
affected by the following
factors :

A
  1. Age
  2. Sex
  3. Altitude of locality
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7
Q

Methods can be used

A
  1. Copper Sulfate or Specific
    Gravity Method
  2. Gasometric Method (
    Oxygen Capacity Method)
  3. Chemical Method ( Iron
    Content Method)
  4. Colorimetric Methods
    A. Visual
    a. Tallquist
    b. Dare
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8
Q

Acid hematin

A

Sahlis Hellige
Haldene
Haden – Hausser
Newcomer
Sahli- adams
Osgood

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9
Q

Alkali Hematin

A

Clegg and king

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10
Q

Photoelectric 2 method used:

A

Oxyhemoglobin method
Cyanmethemoglobin or HiCN
method

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11
Q

best method for measuring plasma
hemoglobin

A

Oxyhemoglobin method

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12
Q

Oxyhemoglobin method

A

20 ul blood + N/10 ammonia soln

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13
Q

the best method considered
by hematologist

A

Cyanmethemoglobin or HiCN
method

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14
Q

Cyanmethemoglobin or HiCN
method used

A

Drabkin’s reagent +20 ul
blood read 540nm

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15
Q

The hemoglobin determination test is used to :

A
  1. Screen for disease associated with anemia
  2. Determine the severity of anemia
  3. Follow the response to treatment for anemia
  4. Evaluate polycythemia
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16
Q

The hemoglobin determination test is used to :

A
  1. Screen for disease associated with anemia
  2. Determine the severity of anemia
  3. Follow the response to treatment for anemia
  4. Evaluate polycythemia
17
Q

Clinical Significance
Increased ( hyperchromia) in:

A

polycythemia
dehydration
in poorly compensated heart disease with cyanosis
changing from high to low altitudes.

18
Q

Decreased ( oligochromia) in

A

Anemias

19
Q

is the presence of free hemoglobin in the plasma.

A

Hemoglobinemia

20
Q

Hemoglobinemia is found in

A

a. severe infection
b. severe burns
c. poisoning with potassium chlorate and
mushroom
d. paroxysmal hemoglobinuria
e. HTR

21
Q

Hemoglobin Determination
( Acid Hematin) material used:

A

Specimen: peripheral or unclotted venous blood
Equipments: hemoglobinometer
Solutions/material: 0.1 N HCl (hydrochloric acid solution), sahli graduated tube, sahli pipette, 20 ul blood, stirring rod, distilled water

22
Q

The cyanmethgb is said to be a method of choice for hemoglobin determination because:

A
  • is stable in dilution
  • standard are readily available
  • all hgb derivatives expect sulfhgb are measure
23
Q

Principle of Acid Hematin Method:

A

Hemoglobin is converted to acid with dilute 0.1 N
HCL and resulting brownish yellow color produced
upon the addition of distilled water with the color
standard in the comparator block.

24
Q

Principle : Cyanmethemoglobin

A

• Hgb iron is converted from ferrous to ferric
state to form methemoglobin by the action of
ferricyanide .
• Methemoglobin then combines with potassium
cyanide to produce a stable
cyanmethemoglobin which is measured
spectrophotometrically

25
Q

Materials of Cyanmethemoglobin

A

• Anticoagulated blood
• Test tubes
• Pipette ( Sahli )/ Automatic
• Spectrophotometer
• Drabkin’s Reagent
• Parafilm
- Drabkin’s Reagent
- Hemoglobin standard

26
Q

Procedure: Cyanmethemoglobin

A
  1. Place 5 cc of Drabkin’s reagent into a test
    tube.
  2. Using the sahli pipette , draw blood to 20 ul
    mark . Wipe the outer wall of the pipette and
    then dispense the blood to the test tube.
  3. Gently draw up and down to rinse the pipette
    and also mix the resulting colloidal suspension.
  4. Cover and mix well by inversion . Let it stand
    for 5 minutes.
  5. Transfer the mixture to a cuvette.
  6. Set the spectrophotometer to at the
    wavelength of 540nm
  7. Continue reading the patient’s and record the result
27
Q

Hemoglobin
Determination method used

A

• Acid Hematin
• Cyanmethemoglobin

AARALIN FOR PRACTICAL CC WEEK 1 (4 decks)

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