Hema Lab Hb And Veni Flashcards
is the red iron-bearing protein
contained within the erythrocytes in
normal blood.
Hb (hemoglobin)
It is the carrier of oxygen from the
lungs to the tissue where it readily
releases this oxygen to the tissues and
then returns to the lungs to combine
with more oxygen.
Hemoglobin
represents the colored
component of blood
Heme or hemoglobin
It is a complex protein
Globin
The molecular weight of one Hb
molecule is
64,458 daltons
Hemoglobin results are
affected by the following
factors :
- Age
- Sex
- Altitude of locality
Methods can be used
- Copper Sulfate or Specific
Gravity Method - Gasometric Method (
Oxygen Capacity Method) - Chemical Method ( Iron
Content Method) - Colorimetric Methods
A. Visual
a. Tallquist
b. Dare
Acid hematin
Sahlis Hellige
Haldene
Haden – Hausser
Newcomer
Sahli- adams
Osgood
Alkali Hematin
Clegg and king
Photoelectric 2 method used:
Oxyhemoglobin method
Cyanmethemoglobin or HiCN
method
best method for measuring plasma
hemoglobin
Oxyhemoglobin method
Oxyhemoglobin method
20 ul blood + N/10 ammonia soln
the best method considered
by hematologist
Cyanmethemoglobin or HiCN
method
Cyanmethemoglobin or HiCN
method used
Drabkin’s reagent +20 ul
blood read 540nm
The hemoglobin determination test is used to :
- Screen for disease associated with anemia
- Determine the severity of anemia
- Follow the response to treatment for anemia
- Evaluate polycythemia
The hemoglobin determination test is used to :
- Screen for disease associated with anemia
- Determine the severity of anemia
- Follow the response to treatment for anemia
- Evaluate polycythemia
Clinical Significance
Increased ( hyperchromia) in:
polycythemia
dehydration
in poorly compensated heart disease with cyanosis
changing from high to low altitudes.
Decreased ( oligochromia) in
Anemias
is the presence of free hemoglobin in the plasma.
Hemoglobinemia
Hemoglobinemia is found in
a. severe infection
b. severe burns
c. poisoning with potassium chlorate and
mushroom
d. paroxysmal hemoglobinuria
e. HTR
Hemoglobin Determination
( Acid Hematin) material used:
Specimen: peripheral or unclotted venous blood
Equipments: hemoglobinometer
Solutions/material: 0.1 N HCl (hydrochloric acid solution), sahli graduated tube, sahli pipette, 20 ul blood, stirring rod, distilled water
The cyanmethgb is said to be a method of choice for hemoglobin determination because:
- is stable in dilution
- standard are readily available
- all hgb derivatives expect sulfhgb are measure
Principle of Acid Hematin Method:
Hemoglobin is converted to acid with dilute 0.1 N
HCL and resulting brownish yellow color produced
upon the addition of distilled water with the color
standard in the comparator block.
Principle : Cyanmethemoglobin
• Hgb iron is converted from ferrous to ferric
state to form methemoglobin by the action of
ferricyanide .
• Methemoglobin then combines with potassium
cyanide to produce a stable
cyanmethemoglobin which is measured
spectrophotometrically
