Helminths Flashcards
Foodborne trematode (Fluke) lifecycle
- Eggs released in faeces
- Miracidia hatch and enter snail
- In snail Miracidia become sporocysts
- In snail - Sporocysts –> Rediae –> Cercariae
- Cercariae swim from snail and encyst on intermediate host
- Cercariae become metacercarie and are ingested by host
- Become adults in gut and migrate to specific organ to lay eggs (hemaphroditic reproduction)
Adult trematode body parts
Leaf shaped
Tegument cover
oral sucker
ventral sucker
blind ending gut
reproductive organ.
Name of liver flukes
Fasciola - hepatica/gigantica
Opisthorchis
Clonorchis - liver/biliary
Name of lung fluke
Paragonimus
Name of gut fluke
Fasciolopsis
Which trematodes have pathogenic eggs?
Schistosomiasis only
Food associated with each foodborne trematode
Fasciola - Aquatic vegetables/ contaminated water
Opisthorchis/Clonorchis - Fish
Paragonimus - Crustaceans
Fasciolopsis - Aquatic vegetables
Fasciola egg description
Egg: 120-150 um
Oval, yellow, operculum.
Fasciola snail
lymnaea truncatula
Opisthorchis egg description
Egg: 27-30um
Yellow, flask shaped, operculum with shoulders
Opisthorchis snail
Bithynia
Symptoms of foodborne trematode infection
Eosinophilia
GI symptoms
Urticaria
Fever
Hepatomegaly and abdominal pain
Weight loss/ malnutrition
Chronic → clinical syndrome from biliary obstruction → RUQ pain, nausea, jaundice.
Cholangiocarcinoma - more common in Opisthorchis/Clonorchis
Extra-heaptic infection → brain, lungs, heart, skin, gut.
Treatment for Trematode infections (including Schistosomiasis)
Praziquantel
(except Fasciola –> give Triclabendazole)
Paragonimus egg description
Egg - 70-95um. Yellow, operculum with shoulders. Unsegmented ovum and mass of yolk cells.
Paragonimus snail
Potodoma/ Semisulcospira
How to kill trematode eggs
cooking/ freezing
Temp >60, <20.
Schistosomiasis - leave water for 24hrs before use.
Schistosomiasis snails
Biomphalaria spp. - S. mansoni
Bulinus spp. - S. haematobium
Oncomelania spp. - S. japonicum (right)
Which sex is more significantly affected by Schistosomiasis and why
Females due to effect on gynecological system, PID and malignancies. Increased risk of catching HIV.
Method of Schistosomiasis innoculation
Any water contact
- via skin
- bathing
- drinking contaminated water
3 types of Schistosomiasis and their locations
S.masoni (GIT) - South America + Africa
S.haematobium (genitourinary) - Africa
S. Japanicum (GIT) - Japan/Asia
How does Schistosomiasis cause disease?
Eggs are potents toxins which stimulate immune reaction and fibrosis. Lead to organ failures, micro-perforations and malignancy
Schistosomiasis lifecycle
- Egg released in faeces (and urine in haematobium and japanicum)
- Eggs hatch in water releasing miracidia which enters snail
- In snail Sporocysts –>Cercariae
- Cercariae swim and penetrate human
- Become Schistosomulae in human and enter bloodstream
- Mature into adults in liver
- Adult worms migrate to venules of bowel/genitourinary tract and lay eggs (sexual reproduction).
Schistosomiasis lifespan
60 years
Early Schistosomiasis symptoms
Cercarial dermatitis “Swimmers itch”
- Acute papular disease
- Occurs from cercariae penetration from skin
- Praziquantel not effective at this stage
Acute schistosomiasis - Katayama fever
- 2-4 weeks post exposure. A serum sickness type illness
- Urticaia, cough, abdo pain, fever.
- Need to treat with Praziquantel twice
S. Haematobium signs/symptoms
Haematuria/ Haematospermia
obstructive uropathy
Increased risk of bladder cancer SCC
Cervical inflammation and cysts/papules
Vaginal bleeding/dyspaeunia
Subfertility and infertility
pulmonary granuloma
S. mansoni signs/symptoms
Bowel changes
Bacteremia
Malignancies
portal hypertension
hepatosplenomegaly
Anaemia
Management of CNS Schistosomiasis
praziquantel and steroids
Population management of Schistosomiasis
- population chemotherapy
- snail control (expensive, toxic)
- water and sanitation (difficult to implement – no def/urine in water).
WHO recommends in endemic countries (>10%) get annual preventative chemo from the age of 2. If the prevalence does not go down, you treat twice yearly. If the prevalence is reduced you can start to switch to a test and treat base.
→ praziquantel: very short half-life. Only adult worms are praziquantel sensitive.
Schistosomiasis diagnostic techniques
Bladder US - characteristic appearance
Stool OCP
Urine filtration + concentration
Serology
Antigen testing - highly specific
Rectal biopsy for eggs
S. Haematobium egg description
100 - 170um with terminal spine
S. Mansoni egg description
100 - 170um with lateral spine
Cestode anatomy
Head (Scolex) - with 4 suckers and Rostellum (line of hooks)
Body segements (Proglottids) in a chain (Strobila) - with last proglottid containing the eggs
Excretory canal
Haemaphroditic reproductive organs
Cestode lifecycle
Proglottids containing eggs
Eggs released in faeces
Ingested by intermediate host
Eggs change into onchospheres
Develops into metacestode in muscle (juvenile) (called cysterceri in taenia and plerocercoid in diphyllobothrium)
Metacestode consumed by definitive host
Metacestode matures to adult in intestine
Cyclophyllidea egg description (pork/beef/dwarf/hydatid tapeworms)
40um size. Round onchosphere containing hooks. Gelatinous outer ring with middle ring called an embryophore.
Difference between Taenia Solium and Taenia Saginata adult tape worms
Solium - more defined head, less uterine branches <15
Saginata - less defined head, more uterine branches 15-30
What organism causes Cysticercosis
The juvenile stage of Taenia Soilum (Pork Tapeworm)
Cysticercosis symptoms
Seizure
Headaches
Visual disturbance
Confusion
Focal neurology
Cysticercosis imaging
rice grain calcification in thighs
hole punch brain (invaginated scolex creating a target shape is diagnostic)
Neurocysticercosis treatment
Albendazole + Dexamethasone.
Add praziquantel in high helminth load.
Dexamethasone alone if raised ICP
Treatment/ Preventative Chemotherapy for Taenia
Praziquentel
Albendazole
Niclosamide
Taenia egg description
Round, 33 to 43 μm with a thick radially striated dark brown wall.
Inside each shell is an embryonated oncosphere with 3 pairs of hooks.
Diagnostics for Taenia
Faecal OCP
Kato-Katz
Less used: PCR, Serology
Intermediate hosts for Diphyllobothrium
Copepod and Fish
(Note usually only single worm infection in human)
Treatment for Diphyllobothrium
Praziquentel
Niclosamide
Description of Diphyllobothrium egg
The egg is pale yellow
-brown and oval in shape, it measures about 65-70 μm.
There is an operculum at one end which can be difficult to see.
The egg contains a mass of granulated yolk cells surrounding an undeveloped ovum
Which Cestode does not require intermediate host
Dwarf Tapeworm (Hymenolepis nana) - However rodents can also be a host