HCT-MTLB Flashcards
Additional information: FUNCTIONS OF THE DANGEROUS DRUGS BOARD - The Dangerous Drugs Board. – The Board shall be the policy-making and strategy-formulating body in the planning and formulation of policies and programs on drug prevention and control.
Noted
WORLD HEALTH ORGANIZATION PUBLISHED STANDARDS FOR:
1. GOOD MANUFACTURING PRACTICE (GMP) 1999
2. GOOD CLINICAL PRACTICE (GCP) 1995
3. GOOD LABORATORY PRACTICE (GLP) 2001
Noted
First clinical laboratory in the Philippines:
San Lazaro Hospital
Research Institute for Tropical Medicine
Manila Public Health Laboratory
National Reference Laboratory
Manila Public Health Laboratory
Introduced medical technology practice in the Philippines after World War II:
Dr. Pio de Roda
Dr. Prudencia Sta. Ana
Dr. Mariano Icasiano
26th Medical Laboratory of the 6th US Army
26th Medical Laboratory of the 6th US Army
RA 5527 consists of:
30 sections
32 sections
50 sections
52 sections
32 sections
Which of the following amends RA 5527 on June 11, 1978?
RA 6138
PD 498
PD 1534
PD 1534
All are sections of RA 5527 amended by PD 1534, except:
Section 3
Section 8
Section 13
Section 18
Section 18
All are qualifications of the Board of Medical Technology, except:
Filipino citizen
Good moral character
Qualified Pathologist, or a duly registered MT
In practice of laboratory medicine or MT for at least 5 years prior to his appointment
Not a member of the faculty of any MT school for at least 2 years prior to appointment
In practice of laboratory medicine or MT for at least 5 years prior to his appointment
Feedback
In practice of laboratory medicine or MT for at least 10 years prior to his appointment.
Board of Medical Technology term of office:
Hold office for ONE (1) YEAR after appointments or until their successors shall have been appointed and duly qualified
Hold office for THREE (3) YEARS after appointments or until their successors shall have been appointed and duly qualified
Hold office for FIVE (5) YEARS after appointments or until their successors shall have been appointed and duly qualified
Hold office for SEVEN (7) YEARS after appointments or until their successors shall have been appointed and duly qualified
Hold office for THREE (3) YEARS after appointments or until their successors shall have been appointed and duly qualified
Minor subject (10%) in the MT Board Exam:
Clinical Chemistry
Hematology
Immunology, Serology & Blood Banking
Clinical Microscopy
Clinical Microscopy
In order to pass the MT examination, a candidate must obtain a general average of at least ___ in the written test.
General average of at least 50%
General average of at least 65%
General average of at least 70%
General average of at least 75%
General average of at least 75%
Refresher course for applicants who have failed the Board Examination for the ___ time.
First time
Second time
Third time
Fourth time
Third time
Certificate of Registration as Medical Technologist is issued to any successful applicant who has attained the age of:
18
19
20
21
21
Revocation:
Unanimous vote (3/3)
Majority vote (2/3)
Unanimous vote (3/3)
Suspension:
Unanimous vote (3/3)
Majority vote (2/3)
Majority vote (2/3)
Which of the following can only be done in a tertiary category laboratory?
Crossmatching
Routine chemistry
Routine hematology
Special hematology
Special hematology
Start of renewal of Clinical Laboratory License:
October 1
November 1
December 1
January 1
October 1
Non–hospital based clinical laboratories shall file applications for renewal of LTO beginning on the FIRST DAY OF OCTOBER UNTIL THE LAST DAY OF NOVEMBER of the current year.
The LICENSE TO OPERATE (LTO) issued to the CLINICAL LABORATORY is valid for:
One year
Two years
Three years
Five years
One year
Acceptable patient identifiers include:
Patient’s name, gender, medical record number
Patient’s name, gender, date of birth
Patient’s name, requesting physician, medical record number
Patient’s name, date of birth, medical record number
Patient’s name, date of birth, medical record number
Acceptable patient identifiers include the patient’s name, an identification number assigned by a health care facility such as the medical record number, or date of birth.
LICENSE TO OPERATE (LTO) Blood Service Facilities is valid for a period of ____ years.
1 year
2 years
3 years
5 years
3 years
According to DOH A.O. 2020-0031, certificate of accreditation of laboratories for DRINKING WATER ANALYSIS is valid for __ year(s) and expires on the last day of December. TAKE NOTE OF YEAR 2020 DOH REVISION.
One year
Two years
Three years
Five years
Three years
Administrative Order No. 2006-0024 known as the “Rules and Regulations Governing the Accreditation of Laboratories for Drinking Water Analysis
Certificate of Accreditation is valid for 2 years and expires on the last day of December
DOH A.O. 2020-0031 (July 17, 2020)
Revised Rules and Regulations Governing the Accreditation of Laboratories for Drinking Water Analysis Laboratory for Drinking Water Analysis (LDWA)
DOH Certificate of Accreditation is valid for 3 years, effective from January 1 of the first year, and shall expire on the last day of December on the third year
Part of drinking water analysis, except:
Calcium
Fecal coliform test
Sodium
Potassium
Potassium
License and accredit drug testing centers in each province and city:
DOH
PDEA
PNP
PRC
DOH
Methylenedioxymethamphetamine (MDMA):
Shabu
Meth
Ice
Ecstasy
Ecstasy
Random drug test:
Applicants for firearm’s license
Officers and members of the military, police and other law enforcers
Students of secondary and tertiary schools
Candidates for public office whether appointed or elected both in the national or local government
Students of secondary and tertiary schools
A drug test is valid for:
Six weeks
Six months
One year
Two years
One year
All results of HIV/AIDS testing shall be confidential and shall be released only to the following persons, except:
Person who submitted himself/herself to such test
Boyfriend of the patient
Either parent of a minor child who has been tested
Legal guardian in the case of insane persons or orphans
Person authorized to receive such results in conjunction with the AIDSWATCH program
Boyfriend of the patient
All results of HIV/AIDS testing shall be confidential and shall be released only to the following persons:
1. Person who submitted himself/herself to such test
2. Either parent of a minor child who has been tested
3. Legal guardian in the case of insane persons or orphans
4. Person authorized to receive such results in conjunction with the AIDSWATCH program
5. Justice of the Court of Appeals or the Supreme Court
The Professional Regulation Commission, otherwise known as the PRC, is a ____-man commission attached to office of the President for general direction and coordination.
Two-man
Three-man
Four-man
Five-man
Three-man
The current (September 2022) PRC CHAIRPERSON is:
Marilyn Barza
Teofilo Pilando
Charito Zamora
Jose Cueto
Charito Zamora
If a medical technologist was not able to complete the number of CPD units upon renewal of PRC license, he may sign a/an:
Affidavit of undertaking
Certificate of exemption
Certificate of registration
Suspension order
Affidavit of undertaking
AFFIDAVIT OF UNDERTAKING
Contains your specific promises to:
1. Undertake to comply with the required number of CPD credit units during the validity of your PRC ID; and to
2. Submit proof of compliance with the required number of CPD units during the next renewal of the PRC ID
An act done to avoid harming the patients:
Beneficence
Non-maleficence
Autonomy
Justice
Non-maleficence
Obligation of MT to the patient:
Compliance to PRC
Compliance to the department of health
Comprehensive health education
Strive for excellence in professional practice
Strive for excellence in professional practice
All traits are mentioned in the MT CODE OF ETHICS, except:
Honesty
Humility
Integrity
Reliability
Humility
Uphold the dignity and respect of my profession and conduct myself a reputation of reliability, honesty and integrity.
Fill in the blank (Code of Ethics): Be dedicated to ____.
Fairness to all and in a spirit of brotherhood toward other members of the profession
Law and shall not participate in illegal work
Responsibilities inherent to being a professional
Use of clinical laboratory science to promote life and benefit mankind
Use of clinical laboratory science to promote life and benefit mankind
Continuing Professional Development Act of 2016:
RA 7719
RA 9288
RA 10912
RA 7170
RA 10912
Published standards for GOOD LABORATORY PRACTICE:
CDC
DOH
PRC
WHO
WHO
WORLD HEALTH ORGANIZATION
PUBLISHED STANDARDS FOR:
1. GOOD MANUFACTURING PRACTICE (GMP) 1999
2. GOOD CLINICAL PRACTICE (GCP) 1995
3. GOOD LABORATORY PRACTICE (GLP) 2001
Components of quality assurance:
Pre-analytical variables
Analytical variables
Post-analytical variables
All of these
All of these
Quality assurance is the broader concept, encompassing preanalytical, analytical, and postanalytical variables.
An example of cellular adaptation is:
Edema
Inflammation
Neoplasia
Dysplasia
Dysplasia
Cellular adaptations:
1. Atrophy
2. Hypertrophy
3. Hyperplasia
4. Metaplasia
5. Dysplasia
Low temperature:
Enhance fixation
Retard fixation
Variable
No effect
Retard fixation
Stopping all cellular activities so that the cells can be viewed under the microscope as if they are still in their original living state:
Decalcification
Embedding
Fixation
Staining
Fixation
Fixative for electron microscopy:
Carnoy’s
Formalin
Glutaraldehyde
Zenker
Glutaraldehyde
Most common and fastest decalcifying agent used:
Formic acid
Hydrochloric acid
Nitric acid
Sulfurous acid
Nitric acid
For most instances, dehydration starts by placing the fixed specimen in:
70% ethyl alcohol
95% ethyl alcohol
Absolute alcohol
Xylene
70% ethyl alcohol
Transition step between dehydration and infiltration with the embedding medium:
Fixation
Clearing
Infiltration
Mounting
Clearing
Enclosed tissue processor:
Mechanical transfer
Dip and dunk
Fluid transfer
Tissue transfer
Fluid transfer
What are the processes (IN ORDER) done by the automatic tissue processor:
Fixation, clearing, dehydration and infiltration
Fixation, infiltration, dehydration and clearing
Fixation, dehydration, clearing and infiltration
Fixation, dehydration, infiltration and clearing
Fixation, dehydration, clearing and infiltration
These knives are used to cut block for ELECTRON MICROSCOPY (EM):
Disposal blades
Steel knives
Diamond or glass knives
Magnetic blades
Diamond or glass knives
Sections fail to form ribbons:
Hard spot in tissue due to calcium
Sections are too thick
Paraffin is impure
Knife edge is dirty
Sections are too thick
Surfaces and edges of the block are not parallel
Horizontal surface of the block is not parallel to the knife
Paraffin wax is too hard
Knife is tilted too much
Sections are too thick
Knife is dull
Effect of basic pH to ripening process:
Slower oxidizing process
More rapid oxidizing process
Variable
No effect
More rapid oxidizing process
pH will have effect on rate of oxidation.
1. Neutral aqueous solution of hematoxylin will form hematein in a few hours
2. Alkaline solutions - more rapid oxidizing process
3. Acid solutions - slower oxidizing process
RETICULIN FIBERS IN GOMORI’S silver impregnation stain:
Black
Blue
Red
Green
Black
A stain containing silver nitrate for demonstration of spirochetes:
Fite-Faraco
Warthin-Starry
Masson-Fontana
Mallory’s PTAH
Warthin-Starry
Frozen sections are stained by hand because:
Staining is more accurate
Prevent overstaining
Faster for one or a few individual sections
Predictable colors
Faster for one or a few individual sections
Frozen sections are stained by hand, because this is faster for one or a few individual sections.
_______ are raised against specific cellular _____ and then conjugated with a _______.
Antigen, antibody, visual marker
Visual marker, antigen, antibody
Antibody, antigen, visual marker
Visual marker, antibody, antigen
Antibody, antigen, visual marker
Gastrointestinal specimens, except:
Gastric lavage
Gastric brush
Fine needle aspirate (submucosal lesions)
Induced vomiting
Induced vomiting
Liquid-based cytology samples:
Body fluids
Touch imprint
Brush sampling
Skin scrape
Body fluids
It is considered to be a most sensitive and specific reagent for lipid staining:
Sudan III
Sudan IV
Sudan Black
Oil Red O
Sudan Black
One measure of the efficiency of a surgical pathology service:
Rapidity of accurate reporting the diagnosis to clinicians
Rapidity of tissue processing
Sufficiency of tissue preservation
Sufficiency of reagents and standards
Rapidity of accurate reporting the diagnosis to clinicians
Objective of a quality assurance program in histopathology:
Ensure to process the tissues
Ensure acceptable service
Ensure to follow standards
Ensure the completeness, accuracy and timeliness of a histopathology report
Ensure the completeness, accuracy and timeliness of a histopathology report
Primary objective in quality and safety control programs in histopathology laboratories:
Correctness of interpretation of reports
Ensure correct sampling
Ensure accurate treatment to patient
Promotion of health and safety of patient, laboratory personnel and environment
Promotion of health and safety of patient, laboratory personnel and environment
Promotion of health and safety of patient, laboratory personnel and environment should be the primary objective in quality and safety control programs adopted by the histopathology laboratories.
Which of the following fixatives contains picric acid, formalin, and acetic acid?
Zenker
Helly
Bouin
Zamboni
Bouin
The volume of fixative should exceed the volume of the tissue by:
1 to 2 times
5 to 10 times
10 to 20 times
25 to 50 times
10 to 20 times
Which of the following fixatives contains formalin, potassium dichromate, and mercuric chloride?
Zenker
Helly
Carnoy
Orth
Helly
Precipitate left in tissues that have been fixed in solutions containing mercuric chloride may be removed by immersion in:
Running water
Sodium thiosulfate
Weak ammonia water
Iodine
Iodine
Commercial stock formaldehyde solutions contain:
4% formaldehyde
10% formaldehyde
37 to 40% formaldehyde
98 to 100% formaldehyde
37 to 40% formaldehyde
Important:
Commercial stock solution: 37 to 40% formaldehyde
As fixing fluid: 10% formalin
Formalin pigment may be removed from tissue by:
Running water
Alcoholic iodine
Alcoholic picric acid
Potassium permanganate
Alcoholic picric acid
To prepare a 10% solution of formalin, which of the following amounts of water should be added to 100 mL of stock formaldehyde:
1,000 mL
900 mL
450 mL
10 mL
900 mL
Important: 1:10 dilution
1 part 40% formaldehyde
9 parts water
TV = 10
Carnoy fluid is prepared with acetic acid, alcohol, and:
Chloroform
Formalin
Acetone
Osmium tetroxide
Chloroform
The first and most important procedure in the preparation of a tissue for microscopic examination is the choice of:
Fixative
Dehydrating agent
Clearing agent
Staining technique
Fixative
Generally, an increase in the temperature of the fixative solution:
Decreases the tissue autolysis
Decreases the fixative penetration
Increases the speed of fixation
Increases the volume of fixative needed
Increases the speed of fixation
Which of the following may cause tissue to become overhardened?
Prolonged fixation
Abbreviated fixation
Inadequate dehydration
Incomplete clearing
Prolonged fixation
To prevent polymerization of formaldehyde, which of the following is added to the commercial stock solutions?
Methyl alcohol
Formic acid
Paraformaldehyde
Sodium phosphate
Methyl alcohol
Which of the following is a dehydrating agent?
Formalin
Xylene
Benzene
Alcohol
Alcohol
A clearing agent for use in processing tissues for paraffin embedding must be miscible with the:
Fixative and paraffin
Dehydrant and paraffin
Fixative and dehydrant
Paraffin and water
Dehydrant and paraffin
Dioxane is a reagent that can be used:
For both fixing and dehydrating tissues
For both dehydrating and clearing tissues
In very small volume ratios
For long periods without changing
For both dehydrating and clearing tissues
A reagent that CANNOT be used for dehydrating tissue is:
Benzene
Absolute alcohol
Dioxane
Acetone
Benzene
Which of the following chemicals is NOT a clearing agent?
Chloroform
Dioxane
Ethanol
Xylene
Ethanol
The process of removing water from tissue is called:
Dehydration
Reduction
Oxidation
Clearing
Dehydration
The dehydration and clearing steps can be omitted when using:
Celloidin
Epoxy resin
Glycol methacrylate
Water-soluble wax
Water-soluble wax
During microtomy, it is noted that most of the tissue is very hard and shrunken. One of the first things to check to prevent its happening in the future is the:
Presence of water in the clearing agent
pH of the fixative
Temperature of the infiltrating paraffin
Freshness of the reagents on the processor
Temperature of the infiltrating paraffin
he temperature of the oven used to maintain a supply of melted paraffin for embedding tissue should be about:
43C
43F
60C
60F
60C
When using a microscope with a x10 ocular and a x40 objective, the total magnification is approximately:
100
400
1,000
4,000
400
Important:
Total magnification (TM)
= magnification of objective x magnification of ocular
= 10 x 40
= 400
The microwave oven creates heat in staining solutions by:
Convection
Conduction
Nonionizing radiation
Electrolytic action
Nonionizing radiation
Harris hematoxylin is used on tissue sections to stain:
Fat
Glycogen
Nuclei
Cytoplasm
Nuclei
Ripening of hematoxylin is a process of:
Hydrolysis
Oxidation
Mordanting
Reduction
Oxidation
The active staining ingredient in ripened hematoxylin solutions is:
Hematin
Hematein
Hematoxylin
Hemosiderin
Hematein
The most important step in regressive hematoxylin staining is:
Postmordanting in picric acid
Use of hematoxylin containing acetic acid
Differentiation in acid-alcohol
Washing in water after the hematoxylin
Differentiation in acid-alcohol
Mordants are used to:
Change the refractive index of the tissue
Help differentiate stains
Link tissue constituents more closely to the dye
Oxidize staining solutions
Link tissue constituents more closely to the dye
The combination of a dye and a mordant is called a/an:
Base
Accelerator
Lake
Buffer
Lake
Mercuric oxide (or sodium iodate) is used in Harris hematoxylin to:
Form hematein
Prevent oxidation
Serve as the mordant
Stabilize the solution
Form hematein
DNA can be demonstrated with:
Eosin
Pyronin
Feulgen reaction
Fast green
Feulgen reaction
The mordant in Weigert hematoxylin is:
Iron
Aluminum
Mercury
Tungsten
Iron
Acetic acid is added to Harris hematoxylin to:
Keep heterochromatin from staining
Make nuclear staining more specific
Ripen the hematoxylin
Form a dye lake
Make nuclear staining more specific
Which of the following is stained rose by the methyl green-pyronin (MGP) technique?
Heterochromatin
DNA
RNA
Golgi apparatus
RNA
Differentiating in the H&E stain is an example of using:
Excess mordant
Weak acid
Oxidizers
Buffers
Weak acid
The Feulgen reaction demonstrates:
DNA only
RNA only
Both DNA and RNA
Phosphoric acid groups
DNA only
Sections for special stains have been accidentally stained with hematoxylin. To remove the hematoxylin, place the sections in:
Acid alcohol
Dilute ammonia
Lithium carbonate
Isopropyl alcohol
Acid alcohol
Glycogen is best demonstrated by the use of:
Crystal violet
Mayer mucicarmine
PAS with and without diastase
Alcian blue with and without hyaluronidase
PAS with and without diastase
Amyloid can be demonstrated with:
Congo red
Mayer mucicarmine
Cresyl echt violet
Alcian blue
Congo red
Which of the following methods best demonstrates elastic tissue?
Verhoeff
Silver impreganation
Gomori trichrome
PAS
Verhoeff
The oil red O stain requires which of the following sections?
Paraffin
Celloidin
Frozen
Plastic
Frozen
The oil red O stain might be used to demonstrate:
Rhabdomyosarcomas
Leiomyosarcomas
Liposarcomas
Adenocarcinomas
Liposarcomas
The best stain for the demonstration of Mycobacterium leprae is the:
Fite
PAS
Kinyoun
Gram
Fite
The PAS reaction will demonstrate fungi, because the cell wall contains:
Lipids
Carbohydrates
Reducing substances
Argyrophilic protein
Carbohydrates
A stain that may be used to demonstrate calcium is the:
Gomori chromium hematoxylin
Fontana-Masson
von Kossa silver nitrate
Schmorl method
von Kossa silver nitrate
The Perl’s stain is used for the detection of:
Reducing substances
Hemosiderin
Hemoglobin
Calcium
Hemosiderin
A tissue frequently used as a control for the Fontana-Masson stain is:
Skin
Lymph node
Liver
Spleen
Skin
Toluidine blue stains:
Helicobacter
Legionella
Spirochetes
Chlamydia
Helicobacter
Acridine orange demonstrates:
Cell wall
Mitochondria
Nucleic acid
Flagella
Nucleic acid
Spirochetes in fixed tissue are best demonstrated by:
Histochemical techniques
Vital staining
Metallic impregnation
Physical methods
Metallic impregnation
Muscle that histologically contains cytoplasmic cross-striations and has multiple nuclei located at the edge of the fibers is classified as:
Smooth
Visceral
Skeletal
Cardiac
Skeletal
The terms “squamous,” “cuboidal,” and “columnar” describe cells that have their origin in which tissue?
Connective
Muscle
Epithelium
Bone
Epithelium
Another name for fat cells is:
APUD cells
Myocytes
Adipocytes
Histiocytes
Adipocytes
Elastic fibers have an affinity for:
Sudan
Indigo
Brazilin
Orcein
Orcein
Transitional epithelium refers to:
Endothelium
Urothelium
Mesothelium
Metaplasia
Urothelium
Which of the following histological features is unique to cardiac muscle?
Cross-striations
Peripherally located nuclei
Intercalated discs
Non-branching fibers
Intercalated discs
In addition to its ability to stain argentaffin cell granules, the Fontana-Masson technique may also be used to stain:
Lipids
Collagen
Melanin
Spirochetes
Melanin
A delicate three-dimensional connective tissue meshwork that forms the framework of organs, such as the spleen and lymph nodes, is made of:
Elastic fibers
Reticular fibers
Smooth muscle
Basement membrane
Reticular fibers
The connective tissue cells actively involved in wound healing are:
Plasma cells
Mast cells
Macrophages
Fibroblasts
Fibroblasts
An example of an exogenous pigment is:
Argentaffin
Melanin
Chromaffin
Carbon
Carbon
The staining method considered to be most sensitive and specific for COPPER is the:
Chloranilic acid
Rhodanine
Orcein
Aldehyde fuchsin
Rhodanine
The nerve process carrying electrical impulses away from the cell body is called a(n):
Neuron
Dendrite
Synapse
Axon
Axon
Dendrites bring electrical signals to the cell body and axons take information away from the cell body.
Hemosiderin, hemoglobin, and bile pigment are classified as:
Endogenous pigments
Artifact pigments
Exogenous pigments
Extraneous pigments
Endogenous pigments
Malignant tumors of connective tissue are known as:
Carcinomas
Lipomas
Sarcomas
Fibromas
Sarcomas
A rhabdomyosarcoma (malignant tumor, skeletal muscle) is suspected in a biopsy submitted to the laboratory. To aid in making a definitive diagnosis, a helpful stain would be the:
Phosphotungstic acid-hematoxylin (PTAH)
Gomori aldehyde fuchsin
Verhoeff-van Gieson
Periodic acid Schiff
Phosphotungstic acid-hematoxylin (PTAH)
The use of mounting media makes stained tissue components more visible by:
Distinguishing only the nuclei
Distinguishing only the cytoplasm
Destaining after hematoxylin
Improving the index of refraction
Improving the index of refraction
Neuritic plaques of Alzheimer’s disease consist of abnormal cell processes often in close proximity to deposits of:
Amyloid
Phospholipids
Neuromelanin
Astrocytes
Amyloid
A disorder involving excess iron deposition in tissues, with resultant TISSUE DAMAGE, is known as:
Hemosiderosis
Hemoglobinemia
Hemophilia
Hemochromatosis
Hemochromatosis