Haemopoiesis

Question 1

What is haemopoiesis

Answer 1

The formation of blood cells

Question 2

Function of neutrophils?

Answer 2

Phagocytosis/acute inflammation

Question 3

Function of eosinophils?

Answer 3

Destroy parasites

Modulate hypersensitivity reaction

Question 4

Function of basophils?

Answer 4

Modulate hypersensitivity reactions

Question 5

Function of monocytes/macrophages?

Answer 5

Modulate immune reactions
Phagocytic clearance
Regulatory functions

Question 6

Function of lymphocytes?

Answer 6

B cells- humoral antibodies
T cells- Regulation and cell mediated immunity
NK cells- Anti viral/tumour

Question 7

Life span of Neutrophils?

Answer 7

7-8 hours

Question 8

Life span of platelets?

Answer 8

7-10 days

Question 9

What is a platelet precursor cell?

Answer 9

Megakaryocyte

Question 10

What is meant by self renewal in haemopoiesis?

Answer 10

A property of stem cells, not present in descendents

Question 11

Which embryological layer are haemopoietic stem cells derived from?

Answer 11

Mesoderm

Question 12

What is the endosteum

Answer 12

The interface between bone and bone marrow

Question 13

What is the difference between red and yellow marrow?

Answer 13

Red is haemopoietically active whereas yellow is fatty and inactive

Question 14

What regulates haemopoiesis?

Answer 14

Complex interplay of intrinsic properties of cells (in particular stem cells)

Transcription factors

Question 15

What factor regulates neutrophil maturation?

Answer 15

Granulocyte-colony stimulating factor (G-CSF)

Question 16

What factor regulates development of megakaryocytes?

Answer 16

Thrombopoietin

Question 17

How are mature cells investigated?

Answer 17

FBC/morphological assessment

Question 18

How are precursor cells investigated

Answer 18

Immunophenotyping
Cytochemistry
Clonogenic assays
Animal models

Question 19

What is immunophenotyping?

Answer 19

Using antibodies to identify patterns of antigen expression to identify a cell type