Haematological malignancies Flashcards
What is the first step in the work up of an haematological disease?
Review of the peripheral blood smear.
How do pathologist define blasts?
- Large cell
- High Nucleus to cytoplasmic ratio
- Immature chromatin
- Big sometimes multiple nucleolous
In a normal bone marrow smear what should the blast count be?
Less the 4.5-5%
What is the best way to gauge a patient’s iron stores?
Look @ the bone marrow.
What proteins do B-Cells express?
- CD45
- CD79a
- CD20
- IgG Kappa
- IgG Lambda
What markers do T-Cells express?
1.CD45
2.CD3/TCR
3.CD7
4.CD4
or
5.CD8
6.CD5
What is the most reliable means of counting particular cell types in bone marrow aspirates?
Flow cytometry
wHAT ARE SOME OF THE LIMITATIONS OF FLOW CYTOMETRY?
- Doesn’t work well for unusually large cells
2. Can’t be immediately correlated with detailed morphological features
What is the most popular marker for maturing granulocytes?
CD33
Reed-Sternberg cells (Hodgkin disease) have what characteristic marker?
CD30
What is required for genotyping?
Cells need to be growing then arrested in metaphase.
How is Fish more convenient than routine karyotyping?
Doesn’t require cells to be growing or arrested in Metaphase.
In AML what are the class I mutations?
Mutations conferring a proliferative advantage.
In AML what are the class II mutations?
Mutations impairing differentiation.
Using immunophenotyping a cell with markers CD34+ would be a?
Blast
