Gtex and Quizzes Flashcards
Tissue with most genetic data
testes
Tissues with least genetic data
blood
Gtex associates
DNA variance with gene expression
tissues analyzed by Gtex found what two categories
tissue specific
gene expressed in all tissues studied
goal of Gtex project
provide a framework for biological interpretation of disease-related variants
what would increase the number of detected loci
sample size and range of tissues
a simplifying assumption made in the field of population genetics is that a new variant (mutation) only arises once. when a new mutation occurs what is the frequency of that variant in the general population
1/2N
Wide but shallow sequencing
allows for more data on different individuals which means more variants
targeted deep exome sequencing
sequencing only the coding regions
less expensive
False discovery rate equation
false over false and true
one of the four hypothesis presented for conceptual framework
individual cell types vary and behave transcriptionally different based on environment, gene expression changes based on cell type and environment it is exposed to
advantages of using nuclei over cells
less mitochondrial DNA
two of three key advancements described when we discussed the historical timelines for the development of single cell technology
nanodroplets
barcoding
when isolating cells or nuclei for single cell or nuclei transcriptome sequencing what is the key starting reagent when starting with tissue in the isolation of each
single cell- proteases
nuclei- detergents
structures used that enable single cell transcriptome profiling of cells or nuclei
Cell barcode
UMI
