Group A Streptoccocus Test Flashcards
Causative agent of group A streptococcal infection?
Streprococcus pyogenes
Two sites for streptococcal infection?
Upper Respiratory Tract and Skin
Most common clinical manifestation of streptococcal infections?
Pharyngitis and Impetigo
This is produced by S. pyogenes cause a rapidly spreading infection deep in the fascia, resulting in ischemia, tissue necrosis, and septicemia if not treated promptly.
Exotoxins
This is the streptococcal infection of the heart?
Rheumatoid Fever
This is the streptococcal infection of the kidney?
Goodspasture Syndrome
These are the immunogenic products produced by streptoccocus mutans?
Streptolysin O, Hyarulonidae, Streptokinase, DNAase, and NADase
What is the less toxic streptococcal antigen between Streptolysin O and S?
Steptolysin O
This is the principle used in Anti-streptolysin O and Anti-DNAse serologic test?
Neutralization
ASO titer usully increases within how many weeks and peaks between how many weeks?
Increase 1-2 weeks
Peak 3-6 weeks
ASO testing usually have 3 stations in its test kit, what do each of these represent?
Positive Control, Negative Control, Serum
If an ASO test kit shows no hemolysis what is the interpretation of the ASO test kit?
Positive, neutralized hemolytic activity
If an ASO test kit shows hemolysis what is the interpretation of the ASO test kit?
Negative, failure to neutralize hemolytic activity
What condition is the patient suspected of with Anti-DNAse B testing?
Glomerulonephrities
In Anti-DNAse B test kit, if the results show a green color, what does this indicate?
Negative, no antibodies to neutralize green color
In Anti-DNAse B test kit, if the results show no color, what does this indicate?
Positive, antibodies produced by streptococcal bacteria neutralized the antigen
This is a screeing test for detection of antibodies to several streptococcal antigens?
Streptozyme Testing
This is the specimen coated in streptococcal antigens used in streptozyme testing?
Sheep’s RBC
test Is based on an immunologic reaction between streptococcal antibodies in the test serum and streptococcal exoenzymes bound to polystyrene latex particles.
ASO Slide Test
This is the ‘animal’ specimen used in anti-streptolysin O that agglutinates between 200-3500 IU/mL?
Horse Sera
Why is plasma not used in streptococcal testing?
Fibrinogen agglutinates with latex particles
What is the specimen ideally used in streptococcal testing?
Fresh clear serum (not lipemic)
In streptococcal testing, a uniform mily suspension with no agglutination within 2 minutes is interpreted as what?
Negative
In streptococcal testing, any observable agglutination within 2 minutes is interpreted as what?
Positive, 200+ IU/mL
If a streptococcal test agglutinates at 5 minutes, what is the interpretation of this?
Positive, nonspecific reaction
