Gram Staining Flashcards
1
Q
Gram Positive
A
Penicillin does work
Thick peptidoglycan layer
2
Q
Gram Negative
A
Penicillin does not work
Lipopolysaccharide layer
Thin peptidoglycan layer
3
Q
1.
A
Cover smear in crystal violet and leave for 1 min
4
Q
2.
A
Flood with iodine solution - acts as a mordant (sticks to specimen)
5
Q
3.
A
Wash of with 70% IDA until washings are pale violet
6
Q
4.
A
Rinse with water
7
Q
5.
A
Flood smear with counter stain (safranin solutions) for 30 secs
