Gram Positive Cocci

Question 1

Test that differentiates Staphylococcus from Streptococcus

Answer 1

Catalase Test

Question 2

Enzyme that has the ability to cause anemia

Answer 2

Hemolysin

Question 3

Purpose of Loeffler’s Medium

Answer 3

enhancement of pigment

Question 4

Tests for differentiation of Staphylococcus and Micrococcus

Answer 4

1. Aerobic growth
2. Anaerobic growth
3. Lysostaphin
4. Bacitracin
5. Modified Oxidase
6. O/F test (Glucose Utilization)
7. Furazolidone

Question 5

O/F test aka

Answer 5

Glucose Utilization

Question 6

organism positive for Modified Oxidase test

Answer 6

Micrococcus luteus

Question 7

Slide coag detects

Answer 7

cell bound/clumping factor

Question 8

Important rule in tube coag test

Answer 8

check every 30 minutes

Question 9

enzyme that possibly dissolves clot

Answer 9

fibrinolysin/ staphylokinase

Question 10

#1 wound infection
#1 osteomyelitis

Answer 10

Staph. aureus

Question 11

S. aureus pigment

Answer 11

Cytochrome/Staphyloxanthin

Question 12

MRSA growth

Answer 12

35’C , 2% NaCl

Question 13

cell wall antiphagocytic virulence of S. aureus

Answer 13

Protein A

Question 14

DNase positive

Answer 14

“SMASH VSS”

Serratia marcesens
Moraxella catarrhalis
Aeromonas
Streptococcus pyogenes
Helicobacter pylori

Vibrio Cholerae
Stenotrophomonas maltophilia
S. aureus

Question 15

DNase methods

Answer 15

A. Toluidine blue = pink zone
Methyl green = clear zone
B. HCl precipitation (+) clear zone; (-) no clearing

Question 16

S. saprophyticus idemtification; Differentiation of Coag (-) Staph

Answer 16

Novobiocin test

Question 17

converts fibrinogen to fibrin clot

Answer 17

coagulase

Question 18

degrades lipids (cellulitis, boils); fat splitting 
enzyme

Answer 18

Lipase

Question 19

degrades hyaluronic acid (cartilage)

Answer 19

Hyaluronidase

Question 20

destroys stratum

granulosum/skin

Answer 20

exfoliatin

-Scalded Skin Syndrome

Question 21

destroys PMNs and monocytes; also

known as Panton Valentine factor

Answer 21

Leukocidin

Question 22

makes Staphylococcus aureus the #1

cause of food poisoning in the Philippines

Answer 22

enterotoxin

Question 23

Duran Reynal Factor

Answer 23

Hyaluronidase

Question 24

MRSA is resistant to

Answer 24

Penicillin & Oxacillin

Question 25

``` G(+) cocci in pairs/chains NON‐MOTILE NON‐SPOREFORMERS Facultative anaerobe Catalase (‐), white “pinpoint” colonies Capnophilic (5‐10% CO2) ```

Answer 25

Streptococcus

Question 26

medium of choice for Stretococcus

Answer 26

Sheep's Blood Agar Plate

Question 27

Selective medium for Stretococcus

Answer 27

(PEA) Phenyl Ethyl Alcohol Agar

Question 28

based on the antigens | (polysaccharides) present on the surface

Answer 28

Lancefield grouping

Question 29

based on | hemolytic reactions

Answer 29

Smith and Brown’s grouping

Question 30

based on temperature

Answer 30

Bergey's

Question 31

Streptococcus specie associated with Colon Cancer

Answer 31

Sterptococcus bovis (non-enterococcus)

Question 32

G(+) diplococci; “lancet‐shape”; | "dome‐shape colonies”

Answer 32

Streptococcus pneumoniae

Question 33

Group A (S. pyogenes) virulence

Answer 33

1. M protein 2. Streptolysin O 3. Streptolysin S 4. Erythogenic toxin 5. Streptokinase 6. Hyaluronidase

Question 34

Differentiates Group D from other Streptococcus; presumptive test for Group D

Answer 34

Bile Esculin hydrolysis test

Question 35

Strep group/s positive for PYRase

Answer 35

``` Group D (Entero) Group A ```

Question 36

CAMP positive organisms

Answer 36

S. agalactiae, Listeria monocytogenes, Helicobacter pylori

Question 37

CAMP stands for

Answer 37

Christine Atkins Munch Peterson

Question 38

Sodium‐Hippurate Hydrolysis Test (+) organism

Answer 38

S. agalactiae

Question 39

Lobar pneumonia / pneumococcal | pneumonia (Community Acquired pneumonia)

Answer 39

S. pneumoniae

Question 40

#1 adult bacterial meningitis

Answer 40

S. pneumoniae

Question 41

SBE (Subacute Bacterial Endocarditis)

Answer 41

Viridans group

Question 42

Tests To Differentiate Streptococcus pneumoniae and | Viridans

Answer 42

"MIBON" ``` Mouse innoculation Inulin Fermentation Bile solubility Optochin/Taxo P Neufeld-Quellung ```

Question 43

detects pneumococcal antibody; skin test detects pneumococcal antibody; skin test

Answer 43

Francis test

Question 44

detects erythrogenic antigen; skin test for | S. pyogenes infection

Answer 44

Dick's test

Question 45

a skin test for scarlet fever that uses antitoxin to the erythrogenic toxin of S. pyogenes subcutaneously;

Answer 45

Schultz‐Charlton Test

Question 46

Schultz‐Charlton Test (+) result

Answer 46

blanching of rash around inectio site

Question 47

Nutritionally variant strep

Answer 47

Streptococcus defectivus Streptococcus adjacens Abiotrophia spp.

Question 48

differentiate | Pediococcus from Streptococcus viridans

Answer 48

Vancomycin resistant test