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Micro - review > Gram Positive Cocci > Flashcards

Gram Positive Cocci Flashcards

1
Q

Test that differentiates Staphylococcus from Streptococcus

A

Catalase Test

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2
Q

Enzyme that has the ability to cause anemia

A

Hemolysin

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3
Q

Purpose of Loeffler’s Medium

A

enhancement of pigment

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4
Q

Tests for differentiation of Staphylococcus and Micrococcus

A
  1. Aerobic growth
  2. Anaerobic growth
  3. Lysostaphin
  4. Bacitracin
  5. Modified Oxidase
  6. O/F test (Glucose Utilization)
  7. Furazolidone
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5
Q

O/F test aka

A

Glucose Utilization

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6
Q

organism positive for Modified Oxidase test

A

Micrococcus luteus

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7
Q

Slide coag detects

A

cell bound/clumping factor

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8
Q

Important rule in tube coag test

A

check every 30 minutes

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9
Q

enzyme that possibly dissolves clot

A

fibrinolysin/ staphylokinase

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10
Q 
#1 wound infection
#1 osteomyelitis
A

Staph. aureus

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11
Q

S. aureus pigment

A

Cytochrome/Staphyloxanthin

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12
Q

MRSA growth

A

35’C , 2% NaCl

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13
Q

cell wall antiphagocytic virulence of S. aureus

A

Protein A

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14
Q

DNase positive

A

“SMASH VSS”

Serratia marcesens
Moraxella catarrhalis
Aeromonas
Streptococcus pyogenes
Helicobacter pylori

Vibrio Cholerae
Stenotrophomonas maltophilia
S. aureus

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15
Q

DNase methods

A

A. Toluidine blue = pink zone
Methyl green = clear zone
B. HCl precipitation (+) clear zone; (-) no clearing

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16
Q

S. saprophyticus idemtification; Differentiation of Coag (-) Staph

A

Novobiocin test

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17
Q

converts fibrinogen to fibrin clot

A

coagulase

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18
Q 
degrades lipids (cellulitis, boils); fat splitting 
enzyme
A

Lipase

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19
Q

degrades hyaluronic acid (cartilage)

A

Hyaluronidase

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20
Q

destroys stratum

granulosum/skin

A

exfoliatin

-Scalded Skin Syndrome

21
Q

destroys PMNs and monocytes; also

known as Panton Valentine factor

A

Leukocidin

22
Q

makes Staphylococcus aureus the #1

cause of food poisoning in the Philippines

A

enterotoxin

23
Q

Duran Reynal Factor

A

Hyaluronidase

24
Q

MRSA is resistant to

A

Penicillin & Oxacillin

25
Q 
G(+) cocci in pairs/chains 
NON‐MOTILE 
NON‐SPOREFORMERS 
Facultative anaerobe 
Catalase (‐), white “pinpoint” colonies 
Capnophilic (5‐10% CO2)
A

Streptococcus

26
Q

medium of choice for Stretococcus

A

Sheep’s Blood Agar Plate

27
Q

Selective medium for Stretococcus

A

(PEA) Phenyl Ethyl Alcohol Agar

28
Q

based on the antigens

(polysaccharides) present on the surface

A

Lancefield grouping

29
Q

based on

hemolytic reactions

A

Smith and Brown’s grouping

30
Q

based on temperature

A

Bergey’s

31
Q

Streptococcus specie associated with Colon Cancer

A

Sterptococcus bovis (non-enterococcus)

32
Q

G(+) diplococci; “lancet‐shape”;

“dome‐shape colonies”

A

Streptococcus pneumoniae

33
Q

Group A (S. pyogenes) virulence

A
  1. M protein
  2. Streptolysin O
  3. Streptolysin S
  4. Erythogenic toxin
  5. Streptokinase
  6. Hyaluronidase
34
Q

Differentiates Group D from other
Streptococcus; presumptive test for Group
D

A

Bile Esculin hydrolysis test

35
Q

Strep group/s positive for PYRase

A 
Group D (Entero)
Group A
36
Q

CAMP positive organisms

A

S. agalactiae,
Listeria monocytogenes,
Helicobacter pylori

37
Q

CAMP stands for

A

Christine Atkins Munch Peterson

38
Q

Sodium‐Hippurate Hydrolysis Test (+) organism

A

S. agalactiae

39
Q

Lobar pneumonia / pneumococcal

pneumonia (Community Acquired pneumonia)

A

S. pneumoniae

40
Q

1 adult bacterial meningitis

A

S. pneumoniae

41
Q

SBE (Subacute Bacterial Endocarditis)

A

Viridans group

42
Q

Tests To Differentiate Streptococcus pneumoniae and

Viridans

A

“MIBON”

Mouse innoculation
Inulin Fermentation
Bile solubility 
Optochin/Taxo P
Neufeld-Quellung
43
Q

detects pneumococcal antibody; skin test detects pneumococcal antibody; skin test

A

Francis test

44
Q

detects erythrogenic antigen; skin test for

S. pyogenes infection

A

Dick’s test

45
Q

a skin test for scarlet fever that
uses antitoxin to the erythrogenic toxin of S. pyogenes
subcutaneously;

A

Schultz‐Charlton Test

46
Q

Schultz‐Charlton Test (+) result

A

blanching of rash around inectio site

47
Q

Nutritionally variant strep

A

Streptococcus defectivus
Streptococcus adjacens
Abiotrophia spp.

48
Q

differentiate

Pediococcus from Streptococcus viridans

A

Vancomycin resistant test

Micro - review (4 decks)

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