GRAM NEGATIVE BACILLI Flashcards

1
Q

H2S producers

A
Salmonella 
Proteus 
Providencia 
Arizona 
Citrobacter freundii 
Edwardsiella
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2
Q

Gram (‐) rods
• Motile w/ peritrichous flagella EXCEPT
Klebsiella, Shigella
• Facultative anaerobe
• Ferments GLUCOSE
• Often produce gas
• Catalase (+) EXCEPT Shigella dysenteriae
• Oxidase (‐) EXCEPT Vibrio, Plesiomonas
shigelloides
• Reduce nitrate to nitrite EXCEPT Erwina,
Pantoea agglomerans

A

Enterbacteriaceae

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3
Q

Antigenic structure

A

Kag, Oag, Hag

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4
Q

capsular antigen; heat labile

*Salmonella ‐ ViAg

A

Kag

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5
Q

somatic Ag; heat stable; IgM

‐ reacts to H antibodies

A

Oag

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6
Q

flagellar antigen; destroyed by alcohols, heat

denaturation; IgG

A

HAg

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7
Q

Sucrose: Lactose: Glucose ratio in TSI

A

10:10:1

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8
Q

H2S indicator in TSI

A

FeSO4

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9
Q

MR (+) control

A

E. coli

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10
Q

MR (-) control

A

Enterobacter cloacae

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11
Q

reagent in Baritts method (VP)

A

α‐napthol and KOH

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12
Q

reagent in Coblentz method (VP)

A

α‐napthol and 40% KOH in creatine

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13
Q

(+) VP

A

” KESH”

Klebsiella
Enterobacter
Serratia
Hafnia

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14
Q

Simmon-Citrate (+) control

A

Klebsiella pneumoniae

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15
Q

Simmon-Citrate (-) control

A

E.coli

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16
Q

Acetate (+) control

A

E.coli

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17
Q

Malonate (+) control

A

Salmonella

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18
Q

Acetamide (+) control

A

Pseudomonas aeruginosa

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19
Q

Rapid Urease

A

“PPM”

Proteus
Providencia
Morganella

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20
Q

Slow Urease

A

“CKEYS”

Citrobater
Klebsiella
Enterobacter gergovae
Yersinia
Serratia
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21
Q

Rapid lactose fermenters enzymes

A

B- galactosidase

Lactose permease

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22
Q

Rapid lactose fermenters

A

“EKE”

Escherichia
Klebsiella
Enterobacter

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23
Q

Late Lactose Fermenters enzyme

A

B- galactosidase

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24
Q

Late Lactose Fermenters

A

”: S2HYCS”

Salmonella arizonae
Shigella sonnei
Hafnia
Yersinia
Citrobacter
Serratia
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25
Non‐lactose Fermenters
All salmonella except S. arizonae All shigella except S. sonnei the rest of enterobacteriaceae
26
rapid test to detect B- galactosidase
ONPG
27
ONPG (+)
Late Lactose Fermenters "S2HYCS"
28
; (‐) control for ONPG
S. typhimurium
29
(+) control for ONPG
E.coli
30
Methyl Umbelliferyl β‐D‐ Glucoronide (MUG) (+) RESULT
Electric blue fluorescens
31
Methyl Umbelliferyl β‐D‐ Glucoronide (MUG) (+)
All escherichia spp. except E. coli O157:H7
32
#1 cause of UTI
Escherichia
33
shigella-like infection
EIEC
34
Cholera-like toxin
ETEC
35
"Childhood diarrhea"
ETEC
36
Virulence test for EIEC
Sereny test
37
HUS causing E. coli
EHEC / VTEC
38
Mucoid colonies on MAC
Klebsiella
39
K. pneumoniae new name
Klebsiella quasipneumoniae subsp. | similipneumoniae
40
Friedlandler's bacillus
Klebsiella quasipneumoniae subsp. | similipneumoniae
41
– causes purulent sinus | infection
K. ozaenae
42
[the only spp. of Klebsiella | that is indole (+)
K. oxytoca
43
only Urease (+) Enterobacter
E. gergoviae
44
i – produces yellow colonies at | 25οC
E. sakazaki
45
Bacterias that are known to cause upper respiratory | tract infections
Klebsiella, Enterobacter, Serratia
46
Serratia | Known to produce these three enzymes:
‐ DNase ‐ Lipase ‐ Gelatinase
47
Serratia pigment
Prodigiosin
48
produces colonies with potato | odor
Serratia odornifera
49
blood bag contaminant, arabinose | +
Serratia liquifascien
50
Salmonella sources of infection:
H2O, dairy products, poultry, pet | house
51
Salmonella on SSA
Black colonies
52
‐ BSA (Bismuth Sulfite Agar) is for
S. typhi
53
Nonmotile Salmonella
Salmonella gallinarum and Salmonella | pullorum
54
(H2S and LDC negative Salmonella
S. paratyphi
55
specimen collection for Salmonella typhi
1st week - Blood and urine 2nd - stool best specimen: bone marrow
56
paratyphoid fever
*Salmonella paratyphi A and B
57
known to cause bacteremia / | septicemia
*Salmonella cholerasuis
58
– known to cause | enterocolitis / gastroenteritis
*Salmonella typhimurium (enteritidis)
59
tube agglutination test for Salmonella
Widal test
60
Widal test antigens
O H VI
61
O antigen
Somatic antigen titer = 1:160 ACTIVE SALMONELLA INFECTION
62
H
Flagellar antigen; past infection / immunity | IgM
63
VI
Capsular antigen; carrier (IgG)
64
Differentiates Shewanella from Salmnonella
Sucrose
65
Inert Bacteria
Shigella
66
only Ornithine (+) Shigella
S. sonnei
67
dysentery
Shigella
68
Culture of Shigella
fresh stool with mucous flecks and rectal | swab of ulcer
69
natural habitats of Shigella
Primates and Humans
70
resembles Salmonella (LDC+)
Citrobacter
71
only specie of Citrobacter that is H2S (+)
C. freundii
72
nursery outbreaks , neonatal meningitis
C. diversus
73
Only ornithine (+) Proteus
P. mirabilis
74
Proteus associated with what renal stone
Triple Phosphate calculi
75
swarming phenomenon on BAP
P. mirabilis
76
P. vulgaris vs mirabilis
Indole test
77
source of OX2 and OX19
P. vulgaris
78
source of OXK (“K” means Kingsbury
P. mirabilis
79
can be isolated from cold and warm blooded animals
Edwardsiella tarda
80
bioterrorism agent
Yersinia
81
Plague Bacillus
Yersinia pestis
82
‐ V and W Antigens
Yersinia pestis
83
‐ causative agent of Bubonic, Pneumonic, Septicemic Plague (Black Death, Famine) ‐ infection of wild rats / rodents
Yersinia pestis
84
vector for Yersinia pestis
Xenopsylla cheopsis
85
stalactite pattern in broth culture
Y. pestis
86
Bipolar bodies
Yersinia Pasteurella -
87
#1 blood bag contaminant
Yersinia enterocolitica
88
bull’s eye appearance / colony
Yersinia enterocolitica
89
CIN (+) but Oxidase (+)
‐ Aeromonas
90
motile at 22◦C but not at 35◦C
Yersinia enterocolitica