ANEROBIC BACTERIOLOGY

Question 1

Collection in anaerobic bacteriology

Answer 1

needle aspiration

Question 2

Reduced media

Answer 2

– Anaerobic BAP,
Schaedler,
Bacteroides
Bile Esculin

Question 3

Anaerobic bact. culture media

Answer 3

Laked Kanamycin Vancomycin BAP (LKVBA)
Anaerobic PEA, 
Egg Yolk Agar, 
Chopped Meat, 
Thioglycollate, 
Lombard Dowell Agar

Question 4

METHODS TO PROMOTE ANAEROBIOSIS

Answer 4

Gas Pak Jar or Mcintosh Fildes jar, Brewer, 
Torbal or any anaerobic jar 
 Cooked meat medium / Chopped Cooked 
Meat Medium 
 Anaerobic Glove Box & chamber 
 PRAS – Pre‐reduced Anaerobically Sterilized 
(Roll Tube of Hungate) 
 Thioglycollate medium

Question 5

Thioglycollate medium

indicator

Answer 5

rezasurin red

Question 6

boiling of thioglycolate broth

Answer 6

drives off O2

Question 7

BRICK RED FLUORESCENECE

Answer 7

Prevotella

Porphyromonas

Question 8

Red fluorescense

Answer 8

Veilonella

Question 9

Pitting of Agar

Answer 9

Bacteriodes ureolyticus

Question 10

Double zone of hemolysis

Answer 10

clostridium perfringens

Question 11

Swarming; Assacharolytic

Answer 11

Clostridium tetani,

Clostridium septicum

Question 12

Molar tooth colonies,

sulfur granules

Answer 12

Actinomyces israelli

Question 13

Breadcrumb colony

Answer 13

Fusobacterium nucleatum

Question 14

Horse manure odor (CCFA)

Answer 14

Clostridium difficile

Question 15

GP Anaerobic bacilli fungus‐like bacteria

Answer 15

Actinomyces

Question 16

GP Anaerobic bacilli lumpy jaw

Answer 16

Actinomyces bovis

Question 17

draining sinus tract with sufur

granules

Answer 17

Actinomyces israeli

Question 18

‐ #1

blood culture contaminant

Answer 18

Propionebacterium acnes

Question 19

vaginitis

Answer 19

Moboluncus

Question 20

GN Anaerobic bacilli needs 20% bile; KVC

(Kanamycin, Vancomycin, Colistin) resistant

Answer 20

Bacteroides fragilis

Question 21

GN Anaerobic bacilli black pigment, red

fluorescence on UVL

Answer 21

. Porphyromonas asaccharolytica

- Prevotella melaninogenica

Question 22

breadcrumb colonies,

fusiform rod

Answer 22

Fusobacterium nucleatum

Question 23

– Vincent’s angina

Answer 23

Fusobacterium necrophorum

Question 24

SPS sensitive, indole

‐

Answer 24

Peptostreptococcus anaerobius

Question 25

Staphylococcus like, Gram positive anaerobic cocci

Answer 25

.Peptostreptococcus niger