ANEROBIC BACTERIOLOGY Flashcards
Collection in anaerobic bacteriology
needle aspiration
Reduced media
– Anaerobic BAP,
Schaedler,
Bacteroides
Bile Esculin
Anaerobic bact. culture media
Laked Kanamycin Vancomycin BAP (LKVBA) Anaerobic PEA, Egg Yolk Agar, Chopped Meat, Thioglycollate, Lombard Dowell Agar
METHODS TO PROMOTE ANAEROBIOSIS
Gas Pak Jar or Mcintosh Fildes jar, Brewer, Torbal or any anaerobic jar Cooked meat medium / Chopped Cooked Meat Medium Anaerobic Glove Box & chamber PRAS – Pre‐reduced Anaerobically Sterilized (Roll Tube of Hungate) Thioglycollate medium
Thioglycollate medium
indicator
rezasurin red
boiling of thioglycolate broth
drives off O2
BRICK RED FLUORESCENECE
Prevotella
Porphyromonas
Red fluorescense
Veilonella
Pitting of Agar
Bacteriodes ureolyticus
Double zone of hemolysis
clostridium perfringens
Swarming; Assacharolytic
Clostridium tetani,
Clostridium septicum
Molar tooth colonies,
sulfur granules
Actinomyces israelli
Breadcrumb colony
Fusobacterium nucleatum
Horse manure odor (CCFA)
Clostridium difficile
GP Anaerobic bacilli fungus‐like bacteria
Actinomyces
GP Anaerobic bacilli lumpy jaw
Actinomyces bovis
draining sinus tract with sufur
granules
Actinomyces israeli
‐ #1
blood culture contaminant
Propionebacterium acnes
vaginitis
Moboluncus
GN Anaerobic bacilli needs 20% bile; KVC
(Kanamycin, Vancomycin, Colistin) resistant
Bacteroides fragilis
GN Anaerobic bacilli black pigment, red
fluorescence on UVL
. Porphyromonas asaccharolytica
- Prevotella melaninogenica
breadcrumb colonies,
fusiform rod
Fusobacterium nucleatum
– Vincent’s angina
Fusobacterium necrophorum
SPS sensitive, indole
‐
Peptostreptococcus anaerobius