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MICROBIOLOGY > Gram Negative Cocci > Flashcards

Gram Negative Cocci Flashcards

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Biology 101 flashcards
1
Q

Neisseria characteristics

A

Non-motile, aerobic, capnophilic

Cold sensitive

non-hemolytic

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2
Q

capnophilic

A

Needs increased CO2

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3
Q

Transport medium

A

Amies with Charcoal, Transgow, JEMBEC (To make sure Neisseria receives increased CO2 continuously)

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4
Q

ferments what?

A

CHO

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5
Q

colonies

A

Develops non-pigmented colonies except; N. flava, N. flavescens, N. subflava

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6
Q

all are catalase and oxidase (+) except

A

N. elongata

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7
Q

Screening test

A

cytochrome oxidase test
- 1% hydrogen peroxide

(+) Result: purple

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8
Q

Cytochrome oxidase test reagent

A

tetramethyl-p-phenylenediamine dihydrochoride (No DMSO)

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9
Q

test to speciate

A

CHO utilization test

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10
Q

CHO utilization test

A

Media: Cystine Trypyticase agar + CHO (glucose, sucrose, etc)

Indicator: phenol red

In an acid pH (+) media will turn YELLOW, (-) is RED

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11
Q

Gramm negative species

A

N. gonorrhoeae
N. meningitidis
M. catarhallis

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12
Q

Neisseria gonorrhoeae characteristics

A
  • never a normal flora, always a pathogen
  • kidney, coffee bean shaped diplococci intracellular with PMN

-

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13
Q

what should be used to collect specimen for

A
  • ryon / dacron fibers

- because it is sensitive to 2 Cs- Calcium aglinate and Cotton swab

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14
Q

Neisseria gonorrhoeae virulence factor

A

pili

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15
Q

rapid test for Neisseria gonorrhoeae

A

Superoxol test - uses 30% hydrogen peroxide

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16
Q

What does Neisseria gonorrhoeae ferment

A

glucose only

17
Q

Neisseria gonorrhoeae causes what

A
  • gonorrhea
  • opthalmia neonatorum ( conjunctival gonorrhea in newborns)
  • PID that causes sterility and perihepatitis/ Fits Hugh Curits syndrome
18
Q

Media used for Cultivating Neisseria gonorrhoeae

A
  • thayer martin
  • modified thayer martin
  • martin lewis
  • new york
  • gc lec
19
Q

Thayer Martin media

A
  • Enhanced Chocolate agar – Heated blood agar
  • Selective for Neisseria
  • Contains VCN
  • Vancomycin - inhibits gram (+)
  • Colistin - inhibits gram (-)
  • Nyastatin – prevents fungi
20
Q

Modified Thayer Martin Media

A

VCN-T

• Trimetophrim lactate – prevents swarming organisms like Proteus

21
Q

Martin Lewis Media

A
  • Contains VCAT

* “A” is Anisomycin – prevent fungi

22
Q

New York City Media

A

Contains VCAT
• “A” is Amphotericin B -prevents fungi
• Can also be used for Mycoplasma hominis and
Ureaplasma urealyticum (urogenital pathogens)

23
Q

GC Lect Medium:

A

Contains VCAT but A is

Amphotericin B and additional LINCOMYCIN – prevents growth of gram (+)

24
Q

Neisseria meningitidis characteristics

A
  • Bean shaped diplococcic; sensitive to SPS
  • Natural habitat is oro and nasopharynx
  • Specimen detect carrier state: nasopharyngeal swab
25
Q

Neisseria meningitidis virulence factor

A

capsule, therefore Neufeld quelling test (+)

26
Q

Neisseria meningitidis what does it ferment

A

maltose and glucose

27
Q

Neisseria meningitidis causes

A

o Bacterial meningitis (5- 29 years old)

o Meningococcemia (bacterial infection of blood)

o Waterhouse–Friderichsen syndrome syndrome (Severe form of Meningococcemia characterized by bleeding of adrenal glands)

28
Q

N.lactamica & N.sicca

A

• N.lactamica: Glucose, Maltose, Lactose (+) & ONPG (+) o ONPG detects late lactose fermenters; (+)
indicator is a yellow color
• N.sicca: Glucose, Maltose, Sucrose (+)

29
Q

Moraxella catarrhalis characteristics

A

• Morphologically and biochemically resembles Neisseria
• Encapsulated, with Pili, non-motile
• Oxidase (+) because of Indophenol oxidase
• Catalase (-) negative
• Gamma hemolytic
• A normal flora of oro and nasopharynx but may cause
otitis media
• Does not degrade sugar – Asaccharolytic / Non
saccharolytic

30
Q

Rapid test for M. catarhalis

A

byurate disk test / tributyrin test positive

+) result: blue color after 5 mins

31
Q

Moraxella catarrhalis

A

Develops HOCKEY PUCK COLONIES – these are colonies

remaining intact when pushed across the plate using inoculating loop

32
Q

Best test to differentiate M. catarrhalis from other Moraxella

A

DNase test

MICROBIOLOGY (9 decks)

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