GPC Conversion of Rt to MW Flashcards
1
Q
3 methods
A
peak position approach
universal calibration
automatic data processing equipment
2
Q
peak position approach
A
known MW polymer used to calibrate column and determine Rt (calibration curve)
same temp, solvent, column
best is unknown = known polymer
3
Q
universal calibration
A
experimentally found that [n]M proportional to Rt
independent of polymer
log([n]M) is a constant for all polymers at same temp, solvent, column
4
Q
if a & k are not available
A
use universal calibration curve and [n] of unknown to determine MW
5
Q
couple GPC to something that…
A
directly measures MW (light-scattering)
