Glycolysis

Question 1

3 steps of Cellular Metabolism

Answer 1

Glycolysis
TCA/Krebs Cycle
Oxidative Phosphorylation

Question 2

6 types of metabolic reactions

Answer 2

Oxidation-Reduction
Ligation requiring ATP cleavage 
Isomerisation
Group Transfer
Hydrolytic 
Addition/Removal of functional groups

Question 3

3 facts about glycolysis

Answer 3

Central to metabolism
Anaerobic process
Occurs in Cytosol

Question 4

Overall reaction for Glycolysis

Answer 4

Glucose + 2ADP + 2Pi + 2NAD+ ——-> 2Pyruvate + 2ATP + 2NADH + 2H+

Question 5

2 main concepts of glycolysis

Answer 5

Invest energy in the form of ATP to form a high energy compound
Produces energy in the form of ATP by splitting a high energy compound

Question 6

Step 1 of glycolysis

Answer 6

Irreversible group transfer
Catalysed by hexokinase
Consumes 1ATP
Forms Glucose-6-Phosphate

Question 7

Step 2 of glycolysis

Answer 7

Isomerisation
Catalysed by phophoglucose isomerase
Forms fructose-6-phosphate

Question 8

Step 3 of glycolysis

Answer 8

Group transfer
Catalysed by phosphofructokinase
Consumes 1ATP
Forms symmetrical fructose-1,6-biphosphate

Question 9

Step 4 of glycolysis

Answer 9

Hydrolytic reaction
Catalysed by aldolase
Gives glyceraldehyde-3-phosphate and dihyroxyacetone phosphate

Question 10

Step 5 of glycolysis

Answer 10

Isomerisation
Catalysed by triose phosphate isomerase
Gives glyceraldehyde-3-phosphate

Question 11

Step 6 of glycolysis

Answer 11

Redox and Group Transfer
Catalysed by glyceraldehyde 3-phosphate dehydrogenase
NAD+ reduced generating NADH
Forms 1,3 biphosphoglycerate

Question 12

Step 7 of glycolysis

Answer 12

Group transfer
Catalysed by phosphoglycerate kinase
Generates 1ATP
Forms 3-phosphoglycerate

Question 13

Step 8 of glycolysis

Answer 13

Isomerisation
Catalysed by phosphoglycerate mutase
Forms 2-phosphoglycerate

Question 14

Step 9 of glycolysis

Answer 14

Group removal (dehydration)
Catalysed by enolase
Forms phosphoenolpyruvate

Question 15

Step 10 of glycolysis

Answer 15

Group transfer
Catalysed by pyruvate kinase
Yields ATP
Forms Pyruvate!