Glycolysis Flashcards
1
Q
What is main product of glycolysis?
A
2 Pyruvate molecules from one glucose
2
Q
How many intermediates?
A
9
3
Q
Why are the intermediates phosphorylated?
A
So that they cannot leave the cell
4
Q
5 steps of preparatory phase
A
- Phosphorylation of C6 to G6P using ATP (Hexokinase)
- Isomerization to F6P (G6P Isomerase)
- Phosphorylation of C1 using ATP (PhosphFruct. kinase- COMMITTING)
- Cleavage into two C3 molecules > DHAP and GADP (Fruct.Bisphosph aldolase)
- Isomerization of DHAP to GADP (isomerase)
5
Q
What is the committing step of glycolysis?
A
Step 3, phosphorylation of F6P to F-1-6-BP utilising 1 ATP
6
Q
What steps of preparatory phase are irreversible?
A
1 and 3
7
Q
What are two products of preparatory phase?
A
Glyceraldehyde phosphate (GADP) and dihydroxyacetone (DHAP)
8
Q
Steps of Payoff phase
A
- Oxidation of GAP to 1,3-bisphosphoglycerate ( reactant+ dehydrogenase) > 1NADH
- Substrate-level phosphorylation yields 1ATP from 1,3-BPG»_space; 3-PG
(PG Kinase) - Migration of phosphoryl group 3-PG»_space; 2-PG
(PG mutase) - 2-PG»_space; Phosphoenolpyruvate (PEP) by enolase
- PEP»_space; Pyruvate with production of 1ATP
9
Q
Products of payoff phase
A
4 ATP
2 NADH
10
Q
Net products of glycolysis
A
2 ATP
2 NADH
2 PYRUVATES
11
Q
Where does glycolysis occur?
A
In the cytoplasm
