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Biochem > Glycolysis > Flashcards

Glycolysis Flashcards

1
Q

rate limiting enzyme for glycolysis

A

phosphofructokinase-1

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2
Q

rate limiting enzyme for fermentation

A

lactate dehydrogenase

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3
Q

rate limiting enzyme for gluconeogenesis

A

fructose-1,6-bisphosphate

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4
Q

glucose enters the cell by…

A

facilitated diffusion or active transport

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5
Q

facilitated diffusion (def.)

A

spontaneous passive transport

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6
Q

active transport (def.)

A

the movement of molecules across a membrane into a region of higher concentration, assisted by enzymes and requiring energy.

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7
Q

fructose 6-phosphate is phosphorylated to…

A

fructose-1,6-bisphosphate using ATP

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8
Q

Hexokinase is inhibited by…

A

glucose-6-phosphate

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9
Q

Glucokinase is induced by…

A

insulin in hepatocytes

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10
Q

Hexokinase has [blank] Km

A

low (reaches maximum velocity at low glucose)

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11
Q

Glucokinase has [blank] Km

A

high

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12
Q

phosphofructokinase-2 (PFK-2) converts…

A

fructose-6-phosphate to fructose-2,6-bisphosphate (F2,6-BP)

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13
Q

glyceraldehyde-3-phosphate dehydrogenase catalyzes…

A

an oxidation and addition of inorganic phosphate (Pi) to its substrate (glyceraldehyde 3-phosphate)

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14
Q

Glyc.Aldeh.Phos.Dehyd. oxidation results in…

A

reduction of NAD+ to NADH and the production of a high energy intermediate (1,3-bisphosphoglycerate)

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15
Q

3-phosphoglycerate kinase transfers…

A

the high energy phosphate from 1,3-bisphosphoglycerate to ADP forming ATP and 3-phosphoglycerate

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16
Q

Substrate-level phosphorylation (def.)

A

ADP directly phosphorylated to ATP using high energry intermediate

17
Q

substrate-level phosphorylation is not dependent on…

A

oxygen and are the only means of ATP generation in an anaerobic tissue

18
Q

in the absence of [blank], fermentation will occur

A

oxygen

19
Q

lactate dehydrogenase will oxidize…

A

NADH to NAD+

20
Q

oxidation of NADH to NAD+ will during fermentation will…

A

replenish the oxidized coenzyme for glyceraldehyde-3-phosphate dehydrogenase

Biochem (11 decks)

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