Glycolysis Flashcards
What does phosphofructose kinase-2 do?
Activates fructose-6-phosphate to fructose-1,6-bisphosphate by activating phosphfructosekinase-1
Which compounds have a higher phosphate transfer ability?
- 1,3-bisphosphoglycerate
- Phosphoenol pyruvate
Why convert glucose to G6P?
Negative charge, traps it inside the cell
What is pyruvate kinase deficiency?
- Lack of the enzyme
- Reduces ability to make ATP
- RBC have short half life
What inhibits and activates step 10?
Inhibited by ATP and acetyl-CoA (high energy)
Activated by high levels of fructose 1,6-bisphosphate (low energy)
What is required in the first step of glycolysis?
- ATP in the form of mgATP
- Hexokinase
What can arsenate do and what does this cause?
- Can substitute for phosphate in step 6
- Is slightly larger than phosphate and forms an unstable 1- arseno, 3-phosphoglycerate
- Breaks doen to form 3PG and skips step 7
- Net ATP is 0
What is tarui disease?
- A defect in PFK
- Accumulation of glycogen
- Breakdown of muscle for energy
When is NAD+ required in glycolysis?
Glyceraldehyde 3-phosphate –> 1,3bisphosphoglycerate by GA3P dehydrogenase
What activates and inactivates step 3?
ATP inactivates
AMP activates
What inhibits glucose to glucose-6-phosphate?
High levels of G6P
What is hexokinase in the liver and what does it do?
Glucokinase
Buffers blood glucose
How is hexokinase different to glucokinase?
Glucokinase has maximum activity higher than hexokinase but much later
