Glycolisis Flashcards
what is the central pathway of glucose metabolism in all cells?
GLYCOLYSIS
When pyruvate is converted into CO2 and H20 glycolysis in this setting is termed ?
aerobic glycolisis
when pyruvate is reduced to lactic acid, glycolysis is termed?
anaerobic glycolysis
which cell in our bodies relies exclusively on blood glucose?
RBC’S
Which cell in our bodies has the lowest rate of ATP synthesis?
RBC’S
How many ATPs are invested to prime the metabolism of glucose?
2 ATP’s
what is the role of GLUT 1 in glycolysis?
Glucose is taken up into the RBC via this facilitated transporter.
how GLUCOSE-6-PHOSPHATE is obtain?
via phosphorylation of glucose
what is the role of enzyme HEXOKINASE?
catalyzed Glucose
Which enzyme helps in the conversion of Glucose 6 phosphate into fructose 6 phosphate?
PHOSPHOGLUCOSE ISOMERASE
Which regulatory enzymes are important for glycolysis?
HEXOKINASE AND PHOSPHOFRUCTOKINASE 1(PFK-1)
Which enzyme determines the critical commitment step in glycolysis?
PFK-1
What is the product after phosphorylating glucose 6 phosphate?
Fructose 6 phosphate F6P
Which enzyme catalyzed the reaction from Fru-6-P into FRUCTOSE 1,6 BIPHOSPHATE?
PHOSPHOFRUCTOKINASE-1(PFK1)
fructose 1,6 BP is cleaved in the middle by which enzyme?
ALDOLASE
dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3 phosphate( GA3P) are the yield products of ?
ALDOLASE REACTION
Which enzyme catalyzes the interconversion reaction of DHAP?
TRIOSEPHOSPHATE ISOMERASE
provide pathway for the investment stage of glycolysis?
glucose-(hexokinase)-glucose 6 phosphate-(phosphoglucose isomerase)- fructose 6 phosphate-( phosphofructosekinase-1)- fructose 1.6 biphosphate.
provide pathway for the splitting stage of glycolysis?
(aldose)-dihydroacetone phostate-(triosephosphate isomerase)-glyceraldehyde 3 phosphate or (aldose)-glyceraldehyde 3.phosphate.
describe substrate level phosphorylation
X-P + ADP—> X+ ATP
When a high energy phosphate compound transfers its phosphate to ADP , yielding ATP is called?
SUBSTRATE LEVEL PHOSPHORYLATION
which enzyme catalyzes a redox reaction, forming a high energy acyl phosphate compound?
GAPDH - Glyceraldehyde 3 phosphate dehydrogenase
after GAPDH Catalyzes the redox reaction we get as a yield product?
1.3-BISPHOSPHOGLYCERATE and coenzyme NAD+ is reduced to NADH
iodoacetamide, p-chloromecuribenzoate and N-ethylmaleimide inhibits which enzyme?
GAPDH
which enzyme catalyzes transfer of the phosphate group from the high energy acyl phosphate of 1.3 BPG to ADP, forming ATP?
Phosphoglycerate kinase (PGK)
Which enzyme converts 3 phosphoglycerate into 2 phosphoglycerate?
PHOSPHOGLYCERATE MUTASE
What kind of enzymes catalyze the transfer of functional groups within a molecule?
MUTASES
2-phosphoglycerate undergoes a dehydration reaction to yield?
PHOSPHOENOLPYRUVATE (PEP)
Which enzyme catalyzes 2-phosphoglycerate ?
ENOLASE
Phosphoglycerate kinase and pyruvate kinase catalyze what kind of reaction?
substrate level phosphorylation reactions
arsenate inhibits what?
substrate level phosphorylation of GAPDH and PGK
Fluoride is used in blood collection to prevent?
False diagnosis of hypoglycemia and acidemia
fluoride inhibits which enzyme?
enolase