Glycogen Metabolism

Question 1

Glucose-6-P Glucose-1-P

Answer 1

Phosphoglucomutase
P-Ser
intramolecular phosphoryl

Question 2

Glucose-1-P + UTP UDP-Glucose + PPi

PPi–> 2Pi

Answer 2

UDP-Glu phosphorphylase / pyrophosphatase
UTP
phosphoanhydride exchange
hydrolysis for the second

Question 3

UDP-glucose + Glycogen —> UDP + Glycogen (+1)

Answer 3

Glycogen Synthase
UDP
glucosyl transfer

Question 4

7- residue fragment linked 1-4 —> new 1-6 linked branch

Answer 4

branching enzyme

transglycosylation

Question 5

Terminal 1-4 linked Glucosyl + Pi —> Glucose-1-P

Answer 5

Glycogen Phosphorylase

phosphorlysis

Question 6

Trisaccharide from 4-residue branch to another branch

Answer 6

debranching enzyme

Question 7

Cleavage of single 1-6 linked Glucosyl —> Glucose

Answer 7

Debranching Enzyme

hydrolysis

Question 8

Reverse of step 1

Answer 8

Phosphoglucomutase
P-Ser
intramolecular phosphoryl transfer

Question 9

Glucose-6-P

Answer 9

1. Glucose-1-P

Question 10

Glucose-1-P + UTP

PPi–>

Answer 10

2. UDP-Glucose + PPi

2. 2Pi

Question 11

UDP-glucose + Glycogen

Answer 11

3. UDP + Glycogen (+1)

Question 12

7-residue fragment linked 1-4–>

Answer 12

4. new 1,6 linked branch

Question 13

Terminal 1-4 linked glucosyl+ Pi–>

Answer 13

5. glucose 1-P

Question 14

Trisaccharide from 4-residue branch to another branch

Answer 14

6.debranching enzyme

Question 15

Cleavage of single 1-6 linked glucosyl–>

Answer 15

7. glucose

Question 16

glucose 1-p

Answer 16

8. glucose 6-P