GGT (Gamma-glutamyltransferase)

Question 1

GGT principle

Answer 1

Gamma-glutamyltransferase catalyzes the transfer of glutamic acid to acceptors like glycyl-glycine in this case.

This process releases 5-amino-2-nitrobenzoate which can be measured at 405 nm.

Question 2

GGT Reagents R1 and R2

Answer 2

✅R1
TRIS
Glycyl-glycine

✅R2
L-Gamma-glutamyl-3-carboxy-4-nitroanilide

Question 3

GGT Normal values Men/Women in SZASZ

Answer 3

According to Szasz
Men: <49 U/L
Women: <32 U/L

Question 4

GGT Reference Range According to IFCC

Answer 4

According to IFCC
Men: <55 U/L
Women: <38 U/L

Question 5

GGT WAVELENGTH

Answer 5

405 nm ( 400-420)

Question 6

GGT Conversion Factor

Answer 6

0.0167 (U/L to ukat)

Question 7

GGT Measuring range and Limit of Detection

Answer 7

✅Measuring range (Serum/Plasma): up to 1200 U/L.

✅Limit of Detection: 6 U/L

Question 8

GGT Clinical Significance and ORIGIN

Answer 8

Hepatobiliary diseases
Bile duct obstruction
Acute pancreatitis
DM
Myocardial infarction
Alcoholism

✅ORIGIN:
LIVER
Brain
Prostate
Pancreas
Kidney

Question 9

Serum /Plasma Interfering substances:

Answer 9

Ascorbic acid — up to 30 mg/dl
Con/UNCONjugated bilirubin — up to 40 mg/dL
Hemoglobin — up to 400 mg/dL
Lipemia — up to 2000 mg/dL