Amylase-Lipase

Question 1

Method of Amylase and Lipase

Answer 1

Amylase - Enzymatic photometric test
Lipase - Enzymatic Color Test

Question 2

Principle Amylase-Lipase

Answer 2

Amylase:

— The substrate 4,6-ethylidene- (G7)-p-nitrophenyl-(G1)-α-D-maltoheptaoside (EPS-G7) is cleaved by α-Amylases into various fragments. These are further hydrolyzed in a second step by α-Glucosidase producing glucose and p-nitrophenol.

Lipase:

— (1,2-o-dilauryl-rac- glycero-3-glutaric acid-(6-methylresorufin) ester) is added to a micro-emulsion which is specifically split by lipase in the presence of colipase and bile acids.

The generated methyl resorufin ester is spontaneously
degraded to methyl resorufin.

Question 3

Reagents Amylase-Lipase

Answer 3

Amylase:
✅R1:
Good’s buffer
NaCl
MgCl2
α-Glucosidase

✅R2:
Good’s buffer
EPS-G7

Lipase :
✅R1:
Good’s buffer
Tauro-desoxy-cholate
Desoxy-cholate
Calcium chloride
Co-lipase (porcine)

✅R2:
Tartrate buffer
Tauro-desoxy-cholate
Color substrate

Question 4

Reference Range Amylase-Lipase

Answer 4

Amylase:

✅Serum/Plasma
Women/Men : <100 U/L or 1.6ukat/L
✅Urine
——-Women: <447 U/L
——-Men: <491 U/L

Lipase:
✅≤ 60 U/L ≤ 1.00 µkat/L

Question 5

Warnings and Precautions amy-lip

Answer 5

✅✅Amylase
The reagents contain sodium azide (0.95 g/L) as preservative. Do not swallow! Avoid contact with skin and mucous membranes

Reagent 1 contains animal and biological material. Handle the product as potentially infectious according to universal precautions and good clinical laboratory practice.

Saliva and skin contain α-Amylases, consequently never pipette the reagents by mouth and avoid skin contact with these reagents.

✅✅Lipase

✅H319 Causes serious eye irritation.
✅P280 Wear protective gloves/protective clothing/eye protection.
✅Reagent 1 contains sodium azide (0.95 g/L) as preservative. Do not swallow

Question 6

Specimen

Answer 6

✅Amylase
1. Serum
2. Heparin plasma
3. URINE

✅Lipase
1. Serum
2. Heparin Plasma

Question 7

WAVELENGTH amy-lip

Answer 7

Amylase — 410/694 nm
Lipase — 571/805 nm

Question 8

Conversion Factor amylase-lipase

Answer 8

Both 0.0167

Question 9

Measuring range/limit of detection: AMYLASE-LIPASE

Answer 9

🌻AMYLASE:
Measuring range (Serum/Plasma): ✅up to 2000 U/L.

Measuring range (Urine): 22 up to ✅4000 U/L.
Limit of Detection:✅ 6 U/L

🌻LIPASE:
Measuringrange: ✅upto300U/L
Limit of Detection: ✅5 U/L

Question 10

Amylase Urine Interfering substances:

Answer 10

Amylase Urine:
● Boric acid✅
● Glucose✅
● Protein✅
● Sodium-Oxalate✅
● Urobilinogen✅

Lipase Interfering subs:
● Ascorbic acid
● Bilirubin (Con/Uncon)
● Hemoglobin
● Lipemia
● N-acetylcysteine✅