Get it together

Question 1

What is the role of fibrinopeptides? 27

Answer 1

increases vascular permeability, and recruits neutrophils/macrophages (results from vascular injury, and leads to hyper acute rejection)

Question 2

What does the kinin cascade do? 27

Answer 2

secretes bradykinin, which acts as a vasodilator, smooth muscle contraction, and vascular permeability increases (results from vascular injury, and leads to hyper acute rejection)

Question 3

For what systems is it okay to match non-ABO Abs?

Answer 3

non-vascularized - cornea, heart valves, and bone

*not stem cell here

Question 4

Microcytotoxicity Test for Preformed Abs

Answer 4

1. Add donor cells to recipient serum (with recipient Abs)
2. Complement is added
3. Dye is added
4. If the MAC forms pores in donor cells, and dye enters then we know that preformed Abs were there

*match it with recipient test to see if they’re a match

Question 5

Microcytotoxicity Test for Class I HLA

Answer 5

1. Add commercial Abs
2. Add complement
3. Add dye
4. If dye gets in to both R and D cells then they’re a match

Question 6

Mixed Lymphocyte Response

Answer 6

1. Add radiation to D cells so they won’t proliferate, but they can serve as APCs
2. Mix with R cells and add 3H-thymidine
3. The radioactivity is incorporated in DNA, and allows proliferation —> means they are NOT a match
   - If radioactivity is not incorporated then R cells don’t proliferate and they are a match

*for HLA II

Question 7

Direct Allorecogniation vs. Indirect

Answer 7

1. R’s T cells recognize stuff from D’s APCs

2. R’s T cells recognize stuff from R’s APCs

Question 8

Mxns of rejection for: hyperacute, acute, chronic

Answer 8

1. Pre existing Abs and complement
2. Th1 cells and CTLs
3. M2 and T cells

Question 9

Filtered Load =

Answer 9

GFR x [plasma] x filterability

Question 10

Free Water Clearance (Ch2o) =

Answer 10

V - Cosm = V - ((Uosm x V) / Posm)

Uosm=urine osm
V= urine flow rate
Posm = plasma osmolarity

Question 11

Obligatory Urine Volume =

Answer 11

amount that must be excreted / 1200 osm/L

Question 12

Oliguria definition for: children, infants, adults

Answer 12

1. <0.5 ml/kg/h
2. <1 ml/kg/h
3. <300-500 ml/kg/h