Genetics-Hershey And Chase

Question 1

What is the purpose of the experiment?

Answer 1

To determine the hereditary material (what molecule passes from parent to offspring?)

Question 2

Materials:

Answer 2

-Bacteriophage
-Bacteria
-Blender
-Centrifuge
-P32
-P31
-S35
-S32

Question 3

Labelled DNA

Answer 3

Expose virus (bacteriophage) to radioactive P32 (DNA will look different when exposed to radioactivity)

Question 4

Labelled protein

Answer 4

Expose capsid (protein coat) of bacteriophage to radioactive S35

Question 5

Experiment number one

Answer 5

Sulfer 35 exposed bacteriophage:
1) Allow virus to infect bacteria
2) Blend bacteriophage to knock off what didn’t physically enter bacteria
3) Centrifuge virus and bacteria (bacteria is at the bottom and bacteriophage at the top)

Question 6

Conclusion experiment number one

Answer 6

Since Sulfur is only in protein and top of test tube is radioactive protein was NOT injected into the bacteria

Question 7

Experiment number two

Answer 7

Phosphorus 32 exposed bacteriophage:
1) Allow virus to infect bacteria
2) Use blender to physically separate virus and bacteria
3) Centrefuge-virus at top bacteria at bottom

Question 8

Conclusion number two

Answer 8

Since phosphorus is only in DNA and bottom of test tube is radioactive, DNA of bacteriophage was injected into the bacteria
.•. DNA must be the genetic material