Genetics 1.6, 3.3, 3.4, 10.1, 10.2 Flashcards
1
Q
When does mitosis occur?
A
- tissue repair
- growth
- embryonic development
- asexual reproduction
2
Q
How is the cell cycle divided?
A
Interphase: G1, S, G2, *G0
Division: mitosis and meiosis
3
Q
What happens in S phase?
A
- DNA replication
- indicator for division
- 2n + c —> 2n + 2c
- chromatin = relaxed chromosome
4
Q
What happens in growth phases?
A
- G1: organelles are duplicating
- G2: cell growth + preparation for mitosis
5
Q
How do chromosomes condense?
A
- condensation needed to pack DNA
- DNA coils to make chromosomes shorter = supercooling
- around histones
6
Q
What happens at the end of G2?
A
- centrosomes (perpendicular centrioles) duplicate
7
Q
What happens in the prophase?
A
- centrosomes move to opposite sides of the cell
- mitotic spindle starts to form
- nucleus disappears
- nuclear membrane breaks down
- DNA coils (very tightly) and separates into individual chromosomes (= metaphase chromosomes)
8
Q
What happens in metaphase?
A
- microtubules attach to centromeres
- kinetochore — protein connected to microtubules (in centromere)
- sends signals when cell is ready to split
- microtubules are put under tension to test attachment
- shortening attachment
- chromosomes line at metaphase pate (cell equator)
9
Q
What happens in anaphase?
A
- centromeres divide
- microtubules shorten
- chromatids are separated and pulled to the opposite sides of the cell
10
Q
What happens in telophase?
A
- separated chromatids form a new nucleus
- nucleolus and nuclear envelope reappears
- chromosomes uncoil
Cytokinesis — cytoplasm divides
11
Q
What is mitotic index?
A
- ratio between cells undergoing mitosis and whole population
- too high indicates cancerogenesis
12
Q
What is the difference between plant and animal cell cytokinesis?
A
- cytokinesis occurs when there are 2 nuclei (telophase)
In animals - plasma membrane pulled inwards - cleavage furrow - accomplished by ring of contractile protein inside plasma membrane - pinches apart
In plants - vesicles move to the equator - fuse and form tubular structures - 2 layers of membrane formed - by exocytosis cells receives pectins
13
Q
What are cyclins?
A
- proteins that control cell cycle
- activating cyclin dependent kinase enzymes (CDK)
- different types of cyclins activating different CDKs
- right concentration of cyclins reached → cell to the next stage
- decreases cancer risk
14
Q
What are CDKs?
A
- enzymes activating proteins into the new stage of cell cycle
- phosphorylation
15
Q
How is cycle regulated?
A
- growth hormones, proteins stimulating quicker division
- checkpoints
- G1: nutrients, growth factor and DNA damage
- G2: cell size
16
Q
What are carcinogens?
A
- chemicals and agents that cause cancer
17
Q
What is a tumour?
A
- abnormal group of cells developing at any stage of life
18
Q
What are mutagens?
A
- set of genes that causes cancer → mutagens
- radiation (UV, X-ray)
- ionising nucleotides in DNA → switch into different nucleotide
- chemicals
- cigarettes ingredients (nitrosamines), metals (As, Ni)
- alcohol increases cell division
- destroys cells which have to recover = higher possibility of mutation
- viruses (HPV, HepC virus)
19
Q
What are protooncogenes and oncogenes?
A
- mutated gene may become oncogene (cancerous gene)
- stimulates cell division and inhibit cell differentiation
- oncogene = protooncogene gains potential to cause cancerogenesis
20
Q
What are different types of tumours?
A
- primary — place in which it occurred first (organs, tissue)
- metastasis = movement of cells from primary tumour to set up secondary
- metastatic tumour — moves onto different tissues (affects multiple organs)
- increased risk of mutation = cancer change
- secondary — place in which cancer develops after moving