Genetic Monitoring and Maintenance

Question 1

First inbred mouse and rat strain?

Answer 1

DBA and PA

Question 2

How many generations of backcrossing is necessary for a congenic strain?

Answer 2

5-10. Standard is 10.

Question 3

How many generations of backcrossing with selection for the donor chromosome is required for a fully consomic strain? Conplastic?

Answer 3

Consomic: 10
Conplastic: 10, with 10 generations of backcrossing inbred females to an inbred male recipient.

Question 4

Question 5

Question 6

How does a skin graft test determine genetic quality control?

Answer 6

Tail skin grafts between animals of same sex, or females to males. If grafts rejected, presence of major or minor histocompatibility differences and thus not the same strain/substrain.

Question 7

As opposed to the skin graft test, what noninvasive postmortem test could be performed to determine genetic similarity?

Answer 7

Morphometry - Mandibular measurements

Question 8

What does current genetic monitoring using DNA rely on?

Answer 8

SNPs - Single nucleotide polymorphisms

Question 9

Question 10

Question 11

Question 12

What is recommended to ensure that an isogenic strain remains truly isogenic to the original parent strain?

Answer 12

Mutant strain background is refreshed by backcrossing to the parental inbred strain approx every 10 generations. Prevents acquisition of background mutations not found in parental strain.

Question 13

Define the congenic interval.

Answer 13

The genome region flanking the introduced allele. Originated from donor strain.

Question 14

What strains make up the Collaborative Cross line?

Answer 14

A/J, C57BL/6J, 129S1/SvIMJ, NOD/ShiLtJ, NZO/HiLtJ, CAST/EiJ, PWK/PhJ, and WSB/EiJ

Question 15

Describe advanced intercross lines.

Answer 15

Maximizes meiotic recombination in the RI population or uses multiple inbred strains to create the hybrid founder animals.