Genetic Information, Variation And Relationships Between Organisms Flashcards
What is the difference between prokaryotic and eukaryotic cells?
Prokaryotes- not membrane bound
Eukaryotes- membrane bound
Describe DNA in prokaryotic cells
Small and circular DNA that is not associated with histones
Describe DNA in eukaryotic cells
One very long DNA molecule, tightly coiled and associated with histone proteins
What does DNA embedded with histones form?
A chromosome
Define a gene
Section of a DNA on a chromosome
What two things do genes code for ?
Polypeptide and functional RNA
What does the sequence of three DNA bases (triplet) code for?
A specific amino acid
What are the three unique things about the genetic code?
Universal (all organisms have the same genetic code)
Non-overlapping (each base is only read once which is only part of one triplet)
Degenerate (one triplet codes for more than one amino acid)
Define exons
The coding section of DNA in pre mRNA
Define introns
The non-coding part of DNA that is spliced to produce mRNA
What is a genome?
Complete set of genes in a cell
What is the structure of molecules in mRNA?
Single stranded polynucleotide
Bases- AUCG
Sugar phosphate backbone
What is the structure of molecules in tRNA?
Clover shaped
Contains a binding site for amino acids
Contains an anticodon complementary to the codon on mRNA
Explain the process of transcription
Takes place in the nucleus as DNA is too big to move out of the nucleus
RNA polymerase attracted to the start codon on DNA
DNA strand is unwound by the DNA helicase forming two strands
RNA polymerase moves down the template strand aligning free floating RNA nucleotides to the DNA
This forms a pre-mRNA strand
As RNA polymerase moves down the strand the template strand will recoil forming the original DNA molecule
This consists of exons and introns which are then spliced out
mRNA is formed and it leaves the nucleus and attaches to a ribosome
In prokaryotes what is the result of transcription?
mRNA- as they do not contain introns that need to be spliced out
In eukaryotes what is the result of transcription?
Pre-mRNA that is then spliced to remove introns and produces an mRNA molecule
Explain the process of translation
Takes place on a ribosome in the cytoplasm
mRNA molecule attaches to a ribosome in which only two codons are present
The cytoplasm contains free floating tRNA molecules that are made up of a specific amino acid and an anticodon that is complementary to a codon on the mRNA strand
tRNA that contains the anticodon for the codon on the ribosomes travels towards the mRNA strand and aligns with it
Peptide bonds are formed with the adjacent amino acids in a condensation reaction
The process continues until a “stop” codon on the mRNA molecule is reached
The amino acid chain (polypeptide) finally forms a protien
Explain the role of ribosomes in translation
Synthesises proteins by translating genetic code that was transcribed in mRNA into an amino acid sequence
Explain the role of tRNA in translation
Translates genetic information into an amino acid sequence that produces a protein
Explain the role of ATP in translation
Provides energy for protein synthesis
What causes a gene mutation?
Changes to the sequence of DNA bases
How do gene mutations arise?
Changes in the sequence of base pairs in DNA molecule that may result in altered polypeptide
What does it mean if a code has a degenerate nature?
More than one triplet codes for the same amino acid