Genetic Fingerprinting Flashcards
1
Q
What is gel electrophoresis used for?
A
to separate the VNTRs
2
Q
What feature of DNA allows gel electrophoresis to work?
A
it is negatively charged and contains different lengths of VNTRs which are unique to individuals
3
Q
What are 2 uses of genetic fingerprinting?
A
- forensic analysis
- parental disputes
- breeding programmes
4
Q
Why is DNA on the nylon membrane treated to form single strands?
A
so that DNA probes can attach/bind
5
Q
List the order of the stages of genetic fingerprinting
A
Extraction
Digestion
Separation
Hybridisation
Development
6
Q
Describe what happens in gel electrophoresis
A
DNA loaded in to wells
Buffer added
Charge passed through agar gel
DNA fragments move towards anode
Transferred to nylon membrane
