Genetic Control of Metabolism Flashcards
Type of chromosome constructed by scientist to act as a vector in DNA technology.
Artificial
DNA ligase
Enzyme used to seal a DNA fragment into a bacterial plasmid.
Genetic variation
Heritable diversity that exists among living things.
Marker
Gene present in a plasmid which enables scientists to determine whether or not the host cell has taken up the plasmid.
Mutagenesis
The creation of mutations.
Mutagenic
Referring to an agent (physical or chemical) that acts on DNA and causes mutations.
Origin of replication
The site on a plasmid which contains genes controlling self replication of plasmid DNA.
Plasmid
Small ring of DNA present in bacteria that is used as a vector in recombinant DNA technology.
Recombinant DNA technology
Process by which genetic material from one organism is inserted into the genome of another.
Restriction endonuclease
Enzyme used to cut DNA into fragments and to cleave open bacterial plasmids that are to receive the DNA.
Restriction site
The location on a plasmid which is cut open by a restriction endonuclease.
Sexual
Form of reproduction by which new genotypes can be produced by fungi but not bacteria.
Sticky end
One of two structures formed at a restriction site when DNA is cut by a restriction endonuclease.
Vector
Agent such as a plasmid by means of which a fragment of ‘foreign’ DNA is inserted into a host cell.