genes recombination and bacteriophages Flashcards
The isolation of genes coding for certain proteins from different species, and
the ability to join them together to form new combinations
genetic recombination
also
genetic engineering
what does the genetic recombinaton require seperation of DNA by
endonucleases
DNA fragments is carried on ?
vector
the DNA fragments is introduced by ?
transformation
what is the vectors ?
the vehicels that carry DNA to the host cell
what is the ideal vectors
-small
-well characterized regarding gene restriction endonucleases cleavage sits
-capable of autonomus replication
-carry slektable marker to distinguish the transformed cells from non transformed cells
the ideal vectors are :
plasmids
bacteriophages
plasmids have in addition to ideal vectors features :
can transfer easily from one cell to another
the bacteriophage can
DNA of phage is used to carry foreign genes by transduction
define viruses
obligate intracellular parsites that only grow on living cell
viruses’ replication depends on :
metabolic energy of host cell
define bacteriophage:
viruses that parasitize bacterial cells.
head of bacterophage contain :
DNA
how the phage attach to the organ
by tail
how does the lytic cycle ends
lysis of the host cell and release of many copies of the phage
where does the absorption occurr
between attachment sites on the phage (tail fibers) and specific receptor sites on
bacteria.
in what stage there is no phage component detected ?
Eclipse
in what stage this happen ?
the viral DNA directs the host cell metabolism to
synthesize new enzymes and new proteins for phage synthesis.
Eclipse
