General Characteristics of Virus

Question 1

Virus is the smallest infectious agent which measures ______ in diameter

Answer 1

20-300nm

Question 2

All virus are HAPLOID except:

Answer 2

Retrovirus

Question 3

Viruses are resistant to antibiotics, True or false?

Answer 3

True

Question 4

Virus are ______ intracellular organisms

Answer 4

Obligate

Question 5

If virus is envelope:

Answer 5

ETHER SENSITIVE / LABILE

Question 6

If virus is naked:

Answer 6

ETHER RESISTANT

Question 7

Enumerate the 6 examples of Naked Viruses

(PRCPPA)

Answer 7

1.Picornavirus
2.Reovirus
3.Calicivirus
4.Papovavirus
5.Parvovirus
6.Adenovirus

Question 8

This is a protein shell or coat that encloses the nucleic acid; either Helical, Icosahedral, Complex

Answer 8

Capsid

Question 9

This is a.k.a. Viral genome / Genomic material; DNA (deoxyribonucleic acid) or RNA (ribonucleus acid) but NOTH BOTH

Answer 9

Nucleic Acid

Question 10

Nucleic acid + Capsid (Enclosed)

Answer 10

Nucleocapsid

Question 11

These are morphological units seen on the
surface of particles.

Answer 11

Capsomeres

Question 12

A lipid containing membrane that surrounds some viral particles.

Answer 12

Envelope

Question 13

A protomers are the smallest functional unit equivalent to building units of the capsid

Answer 13

Structural Units

Question 14

An infectious cycle where in, finding of suitable host cell to invade – viral tropism

Answer 14

Attachment / Adhesion / Adsorption / Invasion

Question 15

The virus attaches to a specific receptor present on the suitable host cell.

Answer 15

Viral Tropism

Question 16

An infectious cycle where in, engulfment of virus through Endocytosis or Direct fusion is called?

Answer 16

Penetration

Question 17

The kind of engulfment for enveloped viruses; Involvement of cytoplasmic vacuoles is called?

Answer 17

Endocytosis

Question 18

The kind of engulfment for naked viruses is called?

Answer 18

Direct Fusion

Question 19

An infectious cycle which is a release of viral content.

Separation of Capsid to genetic material releasing the viral content

Answer 19

Uncoating

Question 20

An infectious cycle which replication and translation occurs is called?

Answer 20

Biosynthesis

Question 21

An infectious cycle which the materials are assembled on a protein coat to enclose the genetic material forming a new cell is called?

Answer 21

Assembly / Maturation

Question 22

An infectious cycle which To the extracellular fluid either thru Budding or Cell lysis is called?

Answer 22

Release

Question 23

What is the appropriate time of collection?

Answer 23

Acute phase reaction within 72 hours of disease

Question 24

Go where the manifestation of the viral disease
shows

Answer 24

Aspirated secretions: maximum recovery

Swabs: use Synthetic Swab (Dacron or Nylon)

Question 25

The transport media for viruses consists of:

Answer 25

buffered isotonic solution + some type of protein + antibiotic

Question 26

Enumerate the transport media for viruses:

Answer 26

Stuart’s
Amies
Leibovitz Emory
Hump’s Balance Salt Solution (HBSS)
Eagle Tissue Culture Medium

Question 27

This is the most widely used for virus isolation (shell vial).

Answer 27

Cell Cultures

Question 28

This is a type of cell cultures that derived from animal tissues; Very expensive

e.g.: Monkey Kidney – excellent for myxovirus

Answer 28

Primary cell culture

Question 29

This is a type of cell cultures that derived from embryonic tissues

e.g., Human embryonic kidney and skin fibroblasts

Answer 29

Semi-continuous cell culture

Question 30

This is a type of cell cultures that derived from Tumor cells / malignant tissues

e.g., HeLa, Vero, Hep2, LLC-MK2, MDCK

Answer 30

Continuous cell culture