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VIROLOGY > General Characteristics > Flashcards

General Characteristics Flashcards

1
Q

Study of viruses

A

Virology

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2
Q

General Characteristics of Virus

A
  • SMALLEST INFECTIOUS agent (20-300 nm)
  • Haploid
  • DNA or RNA
  • obligate intracellular
  • majority of viruses are ENVELOPED
  • Resistant to antibiotics
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3
Q

Viruses are HAPLOID EXCEPT?

A

RETROVIRUSES

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4
Q

ENVELOPED IS ETHER….

A

SENSITIVE/LABILE

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5
Q

NAKED is ether…

A

RESISTANT

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6
Q

NAKED VIRUSES ARE

A

PRCPPA
Picorna
Reovirus
Calicivirus
Papovirus
Parvovirus
Adenovirus

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7
Q

Function of CAPSID

A

Protein shell/coat that encloses nucleic acid

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8
Q

Nucleic acid/Genome material/Viral Genome

A

DNA or RNA

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9
Q

Nucleocapsid

A

Nucleic acid+capsid

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10
Q

Morphological unit seen in surface of particles

A

CAPSOMERES

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11
Q

Capsomeres shapes ranges

A

HELICAL, ICOSAHEDRAL, COMPLEX

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12
Q

Lipid containing membrane that surrounds some viral particles

A

ENVELOPE

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13
Q

3 components of envelope

A

Lipoprotein
Enveloped protein (glycoprotein)
Matrix protein

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14
Q

Structural Unit

A

Protomers

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15
Q

Smallest equivalent to building units of capsid

A

Protomers

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16
Q

INFECTIOUS CYCLE

A

APUBAR
- Adsorption/Attachment/Invasion/Adhesion
- Penetration
- Uncoating
- Biosynthesis
- Assembly/Maturation
- Release

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17
Q

Adsorption

A

Finding of suitable host to invade

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18
Q

VIRAL TROPISM MEANS

A

Virus attaches on a SPECIFIC RECEPTOR ON SUITABLE HOST CELL

19
Q

Engulfment through Endocytosis/Direct Fusion

A

Penetration

20
Q

Engulfment through ENDOCYTOSIS happens in

A

ENVELOPED VIRUS
Involved in Cytoplasmic vacuole

21
Q

Engulfment through DIRECT FUSION

22
Q

Release of viral component; SEPARATION of nucleic acid from capsid

23
Q

Replication and Translation occurs

A

BIOSYNTHESIS

24
Q

Material are ASSEMBLED IN PROTEIN COAT to enclose genomic material forming a new cell

A

Assembly or maturation

25
Q

Release of material in extracellular fluid and repeat the cycle

26
Q

RELEASE happen through

A

Budding or Cell lysis

27
Q

Specimen collection

A

Acute Phase reaction within 72 hours

28
Q

Method of collection for maximum recovery

A

Aspirated secretion

29
Q

Swab uses

A

Synthetic swab such as Dacron/nylon

30
Q

Transport media for virus consist of

A

Buffered isotonic + some type of protein + antibiotic

31
Q

Example of transport media to be use

A

SALEH

Stuarts
Amies
Leibovitz Emory
Eagle Tissue Culture Medium
Half-Balanced Salt Solution

32
Q

Most widely used for virus isolation (shell vial)

A

Cell culture

33
Q

Type of cell culture

A

Primary cell culture
Semi-continuous cell culture
Continuous cell culture

34
Q

Cell culture derived from ANIMALS and is expensive

A

Primary cell culture

35
Q

Example of Primary cell culture

A

Monkey kidney - excellent for MYXOVIRUS

36
Q

Cell culture derived from EMBRYONIC tissues

A

Semi-continuous cell culture

37
Q

Example of Semi-continuous cell culture

A

Human Embryonic Kidney and Skin fibroblast

38
Q

Derived from tumor cells/malignant tissues

A

Continuous cell culture

39
Q

Examples of continuous cell culture

A

HeLa
Vero
Hep2
LLC-MK2
MDCK

40
Q

Immortal cell line derived from CERVICAL CANCER CELLS; oldest and most commonly used human cell line

A

HeLa (Henrietta Lacks)

41
Q

Isolated from KIDNEY EPITHELIAL CELLS from AFRICAN GREEN MONKEY

A

Vero (Verda reno - green kidney)

42
Q

Green monkey sp. name

A

Chlorocebus
Previously Cercopithecus aethiops)

43
Q

Immortalized cell line derived from HUMAN LARYNX CARCINOMA

A

Hep2 (Human Epidermoid 2)

44
Q

LLC-MK2 (Lilly Laboratories Culture-Monkey Kidney 2)

A

Exhibit Epithelial morphology
ISOLATED FROM KIDNEY OF ADULT RHESUS MONKEY

VIROLOGY (3 decks)

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