General Characteristics

Question 1

Study of viruses

Answer 1

Virology

Question 2

General Characteristics of Virus

Answer 2

• SMALLEST INFECTIOUS agent (20-300 nm)
• Haploid
• DNA or RNA
• obligate intracellular
• majority of viruses are ENVELOPED
• Resistant to antibiotics

Question 3

Viruses are HAPLOID EXCEPT?

Answer 3

RETROVIRUSES

Question 4

ENVELOPED IS ETHER….

Answer 4

SENSITIVE/LABILE

Question 5

NAKED is ether…

Answer 5

RESISTANT

Question 6

NAKED VIRUSES ARE

Answer 6

PRCPPA
Picorna
Reovirus
Calicivirus
Papovirus
Parvovirus
Adenovirus

Question 7

Function of CAPSID

Answer 7

Protein shell/coat that encloses nucleic acid

Question 8

Nucleic acid/Genome material/Viral Genome

Answer 8

DNA or RNA

Question 9

Nucleocapsid

Answer 9

Nucleic acid+capsid

Question 10

Morphological unit seen in surface of particles

Answer 10

CAPSOMERES

Question 11

Capsomeres shapes ranges

Answer 11

HELICAL, ICOSAHEDRAL, COMPLEX

Question 12

Lipid containing membrane that surrounds some viral particles

Answer 12

ENVELOPE

Question 13

3 components of envelope

Answer 13

Lipoprotein
Enveloped protein (glycoprotein)
Matrix protein

Question 14

Structural Unit

Answer 14

Protomers

Question 15

Smallest equivalent to building units of capsid

Answer 15

Protomers

Question 16

INFECTIOUS CYCLE

Answer 16

APUBAR
- Adsorption/Attachment/Invasion/Adhesion
- Penetration
- Uncoating
- Biosynthesis
- Assembly/Maturation
- Release

Question 17

Adsorption

Answer 17

Finding of suitable host to invade

Question 18

VIRAL TROPISM MEANS

Answer 18

Virus attaches on a SPECIFIC RECEPTOR ON SUITABLE HOST CELL

Question 19

Engulfment through Endocytosis/Direct Fusion

Answer 19

Penetration

Question 20

Engulfment through ENDOCYTOSIS happens in

Answer 20

ENVELOPED VIRUS
Involved in Cytoplasmic vacuole

Question 21

Engulfment through DIRECT FUSION

Answer 21

Naked

Question 22

Release of viral component; SEPARATION of nucleic acid from capsid

Answer 22

UNCOATING

Question 23

Replication and Translation occurs

Answer 23

BIOSYNTHESIS

Question 24

Material are ASSEMBLED IN PROTEIN COAT to enclose genomic material forming a new cell

Answer 24

Assembly or maturation

Question 25

Release of material in extracellular fluid and repeat the cycle

Answer 25

RELEASE

Question 26

RELEASE happen through

Answer 26

Budding or Cell lysis

Question 27

Specimen collection

Answer 27

Acute Phase reaction within 72 hours

Question 28

Method of collection for maximum recovery

Answer 28

Aspirated secretion

Question 29

Swab uses

Answer 29

Synthetic swab such as Dacron/nylon

Question 30

Transport media for virus consist of

Answer 30

Buffered isotonic + some type of protein + antibiotic

Question 31

Example of transport media to be use

Answer 31

SALEH Stuarts Amies Leibovitz Emory Eagle Tissue Culture Medium Half-Balanced Salt Solution

Question 32

Most widely used for virus isolation (shell vial)

Answer 32

Cell culture

Question 33

Type of cell culture

Answer 33

Primary cell culture Semi-continuous cell culture Continuous cell culture

Question 34

Cell culture derived from ANIMALS and is expensive

Answer 34

Primary cell culture

Question 35

Example of Primary cell culture

Answer 35

Monkey kidney - excellent for MYXOVIRUS

Question 36

Cell culture derived from EMBRYONIC tissues

Answer 36

Semi-continuous cell culture

Question 37

Example of Semi-continuous cell culture

Answer 37

Human Embryonic Kidney and Skin fibroblast

Question 38

Derived from tumor cells/malignant tissues

Answer 38

Continuous cell culture

Question 39

Examples of continuous cell culture

Answer 39

HeLa Vero Hep2 LLC-MK2 MDCK

Question 40

Immortal cell line derived from CERVICAL CANCER CELLS; oldest and most commonly used human cell line

Answer 40

HeLa (Henrietta Lacks)

Question 41

Isolated from KIDNEY EPITHELIAL CELLS from AFRICAN GREEN MONKEY

Answer 41

Vero (Verda reno - green kidney)

Question 42

Green monkey sp. name

Answer 42

Chlorocebus Previously Cercopithecus aethiops)

Question 43

Immortalized cell line derived from HUMAN LARYNX CARCINOMA

Answer 43

Hep2 (Human Epidermoid 2)

Question 44

LLC-MK2 (Lilly Laboratories Culture-Monkey Kidney 2)

Answer 44

Exhibit Epithelial morphology ISOLATED FROM KIDNEY OF ADULT RHESUS MONKEY