General Flashcards
What does the Michaelis menten equation describe
How rxn velocity varies with substrate concentration
MM kinetics follow a ___ curve?
Hyperbolic curve
What are factors wc can affect the rxn rate
Substrate conc, temp, pH
Lineweaver burke plot is used for
Calculated Km and Vmax; determines mechanism of action of enzyme inhibitors
Precursor of arachidonic acid
Linoleic acid
Alcohol+ diacylglycerol/ spingosine via phosphodiester bond
Phospholipid
Spingosine and Fatty acid
Ceramide
Ceramide and gluctose or galactose
Cerebroside
Ceramide and oligosaccharide
Globosidr
Ceramide and N acetylneuramic acid
Ganglioside
Ceramide and sulfated galactose
Sulfatides
3 main kinds of eicosanoids
Porstaglandin, thromboxane, leukotrienes
Amino acid structure
Caboxyl, amino group, unique side chain
Smallest amino acid
Glycine
Largest amino acid
Tryptophan
Amino acid used in the 1st step of heme synthesis
Glycine
Major inhbitory NT in spinal cord
Glycine
AA that carries N from peripheral tissues to the liver
Alanine
Precuror of niacin, serotonin and melatonin
Tryptophan
Precursor for homocysteine
Methionine
An imino acid
Proline
To cysteines form
Custine
Major carrier of nitrogen from the peripheral tissues to the liver
Glutamine
Precursor for GABA and glutathione
Glutamate
Used in diagnosis of folic acid deficiency
N- formiminoglutamate excretion test
Precursor of creatinine, urea and nitric oxide
Arginine
All amino acids are chiral except for
Glycine
Chemical compound that has a total net charge of zero
Zeitteriob
Condotionally non essential amino acids
Arginine, histidine
Most common secondary structure of proteins
Alpha helix
Minor transporternof hgb in bloof
Hemoglobin (carbaminoHb)
HgB form with high oxygen affinity? Taut or Relaxed
Relaxed
Heme protein in heart and skeletal muscle
Myoglobib
Curve followed by Hgb
Sigmoidal
Deoxy form of HgB has a greater affinity for protons than does oxyhemoglobin
Bohr effect
Stabilizes T structure of Hgb by forming additional salt bridges that must be broken prior to conversion to the R state
2,3 BPG
Oxidized form of Hgb (Fe3)
Methemoglobin
Chocolate cyanosis
Methemoglobinemia
Most abundant protein in body
Collagen
Most common form of collagen
Type I
Amino acid sequence of collagen
Gly-X-Y ; x: proline= kinking; y: hydroxyproline or hydroxylysine
HCl is produced by what cells
Parietal cells
Pepsinogen is produced by
Chief cells
All amino acids must transfer their amino groups to a ketoglutarate to from GLUTAMATE except
Lysine and threonine
Coenzyme in transamination
Vit B6
Removal of excess N from peripheral tissues is via
glutamine, alanine, glutaminase
Also known as the Krebs Henseleit Cycle/ Ornithine cycle
Urea cycle
How do enzymes work
Lower free energy of activation