Genentech Flashcards
1
Q
parameters to monitor bioreactor
A
pH,
temperature,
agitation,
oxygen concentration
2
Q
parameters to monitor cell growth
A
microscopic count, check absorbance at spectrophotometer, generate growth curve, spread plates, wet weight
3
Q
cGMP
A
number one goal of pharmaceutical companies,
minimum regulations enforced by the FDA,
inspections at least every 2 years,
involves storage and handling as well
4
Q
SOP
A
has to be closely followed
5
Q
buffer
A
solutions that keeps pH stable
6
Q
pH
A
how acidic or alkaline a solution is
7
Q
isoelectric point
A
pH at which a particular molecule carries no net electrical charge
8
Q
protein
A
macromolecules composed of long chains of amino acids
9
Q
four types of chromatography
A
size exclusion,
ion exchange,
affinity,
hydrophobic interaction
10
Q
bioreactor
A
apparatus to grow organisms under controlled conditions, used to produce vaccines, antibodies, etc.
