gene technologies

Question 1

what is a palindrome sequence?

Answer 1

sequences consisting of antiparallel base pairs

Question 2

what does reverse transcriptase do?

Answer 2

makes DNA from RNA. the DNA produced in making DNA fragments from mRNA is called cDNA

Question 3

what do restriction endonucleases do?

Answer 3

enzymes that recognize specific palindromic sequences and cut the DNA at these places

Question 4

give 3 examples of mutagenic agents

Answer 4

1. ACTING AS A BASE-chemicals called base analogs can substitute for a base during DNA replication
2. ALTERING BASES-some chemicals can delete or alter bases
3. CHANGING THE STRUCTURE OF DNA-some types of radiation can change the structure of DNA which causes problems during DNA replication

Question 5

what are the two types of gene that control the rate of cell division?

Answer 5

• tumor suppressor gene

- proto-oncogenes

Question 6

name the two types of gene therapies and the techniques used to perform them

Answer 6

gene replacement-somatic cell therapy

gene supplementation-gene line therapy

Question 7

what is a vector?

Answer 7

a vector is something that is used to transfer DNA into a cell

Question 8

what is the role of DNA ligase?

Answer 8

DNA ligase joins the sticky ends of the DNA fragments to the sticky ends of the vector DNA