Gene Tech - Production of Fragments Flashcards

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1
Q

What are the ways that DNA fragments can be produced?

A
  • Reverse transcriptase
  • Restriction endonuclease
  • Gene Machine
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2
Q

How is reverse transcriptase used to form DNA fragments?

A
  • mRNA coding for the desired gene is isolated.
  • Reverse transcriptase is used to form cDNA by using mRNA as a template.
  • mRNA detaches from cDNA through hydrolysis using an enzyme.
  • DNA polymerase is used on the template cDNA to form double stranded DNA.
  • This has a copy of the desired gene.
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3
Q

What is cDNA?

A

Single stranded complementary copy of DNA

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4
Q

How are restriction enzymes used to produce DNA fragments?

A
  • Cut a DNA double strand at recognition sites.
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5
Q

Where does reverse transcriptase come from?

A

Retroviruses

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6
Q

How does the gene machine produce DNA fragments?

A
  • The desired sequence of DNA is worked out from a database.

- The mRNA codons are found and the complementary DNA is worked out.

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