Gene Tech - Production of Fragments Flashcards
1
Q
What are the ways that DNA fragments can be produced?
A
- Reverse transcriptase
- Restriction endonuclease
- Gene Machine
2
Q
How is reverse transcriptase used to form DNA fragments?
A
- mRNA coding for the desired gene is isolated.
- Reverse transcriptase is used to form cDNA by using mRNA as a template.
- mRNA detaches from cDNA through hydrolysis using an enzyme.
- DNA polymerase is used on the template cDNA to form double stranded DNA.
- This has a copy of the desired gene.
3
Q
What is cDNA?
A
Single stranded complementary copy of DNA
4
Q
How are restriction enzymes used to produce DNA fragments?
A
- Cut a DNA double strand at recognition sites.
5
Q
Where does reverse transcriptase come from?
A
Retroviruses
6
Q
How does the gene machine produce DNA fragments?
A
- The desired sequence of DNA is worked out from a database.
- The mRNA codons are found and the complementary DNA is worked out.
