Gene Regulation

Question 1

Transcriptional Control

Answer 1

Presence of DNA motif in promoter

Promoter accessibility

Abundance and activity of TF

Question 2

Signal Transduction

Answer 2

Process of conveying info from extracellular signal to intracellular target

Question 3

Steroid Hormones

Answer 3

Lipid hormones

Pass through membrane and bind intracellular receptors

Enters nucleus and binds to hormone response elements (HRE) present as enhancers in hormone regulated gene

Question 4

Peptide Hormones

Answer 4

Extracellular hormone bind to membrane receptors

Signal transduction leads to TF activation

Question 5

Properties of Enhancers

Answer 5

Act over large distances

Orientation independent

Position independent (can be upstream, downstream, in intron…etc)

Question 6

Domain

Answer 6

Autonomously folding functional module of a protein usually with several motifs

Question 7

Motif

Answer 7

Pattern in a protein structure associated with its secondary structure

Question 8

DBD

Answer 8

Domain binding domain

TF bind to promoter/enhancer here

Question 9

TAD

Answer 9

Transcriptional activation domain

Question 10

DNA binding motif in TF

Answer 10

Helix turn helix

Zinc finger

Question 11

Protein Dimerization motif in TF

Answer 11

Leucine zipper

Helix loop helix

Question 12

ChIP-seq

Answer 12

Sequence chromatin fragment

Question 13

ChIP-chip

Answer 13

○ Hybridize pulled down sequence with fluorescent nucleotide

See which spot it binds to

Question 14

Semi-quantitative ChiP

Answer 14

Analyze PCR products by gel electrophoresis