Recombinant Plants and Animals

Question 1

Codon bias

Answer 1

Humans have bias over codons they want to use compared to plants or other organisms

i.e. E Coli might prefer CCC to CCG, mutating codon to CCC would result in higher expression

Question 2

Inclusion Bodies

Answer 2

Prevents proteins from folding properly

Solution- fold protein isolated from inclusion bodies

Question 3

Forward Genetics

Answer 3

Begins with mutant phenotype and attempts to identify the mutant gene responsible for the phenotype

Question 4

Reverse Genetics:

Answer 4

• Begins with a gene and attempts to identify its function by mutating it, and looking for a change in phenotype
i..e Knock out

Question 5

T-DNA Insertions

Answer 5

Collection of transgenic plants with genes disrupted due to T-DNA insertions