Gene Regulation 2 Flashcards
1
Q
basal promoter
A
- binds to TFs required for recruitment of RNA polymerase and transcription initiation
2
Q
enhancer sequence (2)
A
- regulatory DNA sequences that, when bound by TFs, enhance the transcription of an associated gene b regulating time, place and transcription amount
- position and orientation independent and can be far away from associated gene
3
Q
boundary elements/insulators
A
- prevent enhancer form activating other promoters
4
Q
enhancer trap
A
- reporter gene with a promoter, but lacking any enhancer elements
5
Q
promoter trap
A
- a reporter gene having no promoter
6
Q
use of enhancer/promoter traps (2)
A
- identify enhancers and promoters that function in different tissues or time of development to use in engineering the expression of other genes
- identify genes transcribed in various spatial or temporal patterns during development
7
Q
control of transcription factors (3)
A
- transcriptional regulation
- post-transcriptional regulation
- post-translational regulation
8
Q
post-transcriptional regulation examples (4)
A
- alternative splicing
- RNA localization
- inhibition of translation (miRNA)
- poly A tail and 5’-cap added for stability
9
Q
post translational regulation (5)
A
- phosphorylation
- protein degradation
- proteolysis
- allosteric regulation
- protein localization
