Gene Expression: Translation Flashcards
What is translation?
synthesis of polypeptides according to the amino acid sequence directed by the sequence of codons in mRNA
What is each tRNA made from?
single transcript produced by RNA polymerase III
What is the basic structure of every tRNA?
stem-and-loop structure stabilized by hydrogen bonds between bases on neighboring segments of RNA chain
What is the anticodon?
region of tRNA that is responsible for recognizing the mRNA codon to be translated
What is the amino acid acceptor site?
region of tRNA that attaches the amino acid to be brought to the ribosome
What nitrogenous base plays a critical role in wobble base pairing?
inosine
What does the Wobble Hypothesis state?
the first two codon-anticodon pairings obey normal base pair rules but the third position is more flexible
What provides the energy to attach an amino acid to a tRNA?
hydrolysis of two high energy phosphate bonds
What is tRNA loading?
amino acid activation; process of reaction coupling to bind an amino acid to a tRNA molecule
What does the breaking of the bond between the amino acid and tRNA molecule drive?
peptide bond formation
What is the function of aminoacyl-tRNA synthetase enzymes?
specific to each amino acid and aids in binding the correct amino acid to the correct tRNA
What are the two functions that amino acid activation serves?
- accurate amino acid delivery
- thermodynamic activation of the amino acid
What is the unit of measurement for the ribosome subunits?
Svedberg unit
What is the Svedberg unit?
sedimentation rates; how quickly something will sink in a gradient during centrifugation
What are the sizes of the prokaryotic and eukaryotic ribosomes?
prokaryotic = 70S
eukaryotic = 80S