Gene Expression: Translation Flashcards

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1
Q

What is translation?

A

synthesis of polypeptides according to the amino acid sequence directed by the sequence of codons in mRNA

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2
Q

What is each tRNA made from?

A

single transcript produced by RNA polymerase III

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3
Q

What is the basic structure of every tRNA?

A

stem-and-loop structure stabilized by hydrogen bonds between bases on neighboring segments of RNA chain

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4
Q

What is the anticodon?

A

region of tRNA that is responsible for recognizing the mRNA codon to be translated

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5
Q

What is the amino acid acceptor site?

A

region of tRNA that attaches the amino acid to be brought to the ribosome

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6
Q

What nitrogenous base plays a critical role in wobble base pairing?

A

inosine

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7
Q

What does the Wobble Hypothesis state?

A

the first two codon-anticodon pairings obey normal base pair rules but the third position is more flexible

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8
Q

What provides the energy to attach an amino acid to a tRNA?

A

hydrolysis of two high energy phosphate bonds

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9
Q

What is tRNA loading?

A

amino acid activation; process of reaction coupling to bind an amino acid to a tRNA molecule

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10
Q

What does the breaking of the bond between the amino acid and tRNA molecule drive?

A

peptide bond formation

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11
Q

What is the function of aminoacyl-tRNA synthetase enzymes?

A

specific to each amino acid and aids in binding the correct amino acid to the correct tRNA

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12
Q

What are the two functions that amino acid activation serves?

A
  1. accurate amino acid delivery
  2. thermodynamic activation of the amino acid
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13
Q

What is the unit of measurement for the ribosome subunits?

A

Svedberg unit

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14
Q

What is the Svedberg unit?

A

sedimentation rates; how quickly something will sink in a gradient during centrifugation

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15
Q

What are the sizes of the prokaryotic and eukaryotic ribosomes?

A

prokaryotic = 70S
eukaryotic = 80S

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16
Q

What are the three binding sites of the ribosome?

A

A site
P site
E site

17
Q

What is the A site of the ribosome?

A

where each new tRNA delivers its amino acid

18
Q

What is the P site of the ribosome?

A

where the growing polypeptide , still attached to the tRNA, is located during translation

19
Q

What is the E site of the ribosome?

A

now-empty tRNA sits prior to its release from the ribosome