Gene Expression Flashcards
What does Gene expression involve in DNA sequences
It involves the Transcription and Translation of DNA sequences
How much here’s in a cell are expressed
Only a fraction
What types of RNA does transcription and translocation involve
mRNA
tRNA
rRNA
What is the structure of RNA
RNA is single stranded and made up from RNA nucleotides
What does a RNA nucleotide contain
Phosphate,Ribose sugar and base
What are the RNA bases and what does each base pair to
Adenine-Uracil
Guanine- Cytosine
Differences in RNA and DNA
RNA has one strand where as DNA has two
Adenine in RNA is complimentary to Uracil where as in DNA adenine is complementary to Thymine
In RNA the sugar present in nucleotides is Ribose sugar where as in DNA it is deoxyribose sugar
Types of RNA where it’s found in the cell and what’s it’s job
mRNA is found in the nucleus and it’s transcribed from DNA. mRNA is translated by ribosomes in the cytoplasm to make proteins
tRNA is found in the cytoplasm/ribosomes each tRNA molecule carries its specific amino acids to the ribosome during translocation. Amino acids are joined to make a protein.
rRNA is found in the ribosome Ribosomal RNA and proteins form the ribosome
Overview of Transcription and Translation
mRNA is transcribed from DNA in the nucleus and translated into protein by the ribosomes in the cytoplasm
What does transcription involve
RNA polymerase moves along the
DNA unwinding the double helix and
breaking the hydrogen bonds
between the bases of the DNA
coding for the gene to be expressed
Free RNA nucleotides move towards
the expose bases of one strand &
form complementary base
pairs. (uracil pairs with adenine &
guanine with cytosine).
RNA polymerase synthesises a
primary transcript of mRNA from
RNA nucleotides by complementary
base pairing.
What is each triplet of bases on a mRNA called
A codon
What is an intron
An intron is a non coding region of DNA
What is an exon
An exon is a coding region of DNA
What is RNA splicing
RNA splicing forms a mature mRNA transcript. The introns of the primary transcripts are non-coding regions and are removed. The exons are coding regions and are joined together to form the mature transcript. The order of the exons are unchanged during splicing.
Translocation involves another type of RNA what type of RNA is it
tRNA