Gene Editing Steps

Question 1

1. Preparing to edit

A DNA-cutting enzyme called _ forms a complex with a short piece of RNA that has been designed to match a specific DNA sequence

Answer 1

Cas9

Question 2

2. Search

The complex (cas9) moves along the DNA until it finds the _ on patients genome, which it attaches itself

Answer 2

Matching sequence

Question 3

3. Cut

Cas9 enzyme cuts DNA at _

Answer 3

Exactly desired point in the patients genome

Question 4

3. Cut

Once cut is made, _ depart (2)

Answer 4

Guide RNA

Cas9 enzyme

Question 5

4. Paste

A copy of _ is also present

Answer 5

Replacement DNA

Question 6

4. Paste

Cell uses its own repair capabilities to _

Answer 6

Insert copy of replacement DNA in place