Gel Electrophoresis

Question 1

how does electrophoresis work

Answer 1

opposites attract - anode and cathode at each end
DNA moves to anode, movement proportional to m/z

Question 2

Gels

Answer 2

• integrated fibrils act as a selective medium –> larger molecules hindered more
• compact DNA moves faster that rigid rod DNA helix
• can have agarose or PAGE
• dyes added for visualisation can be fluorescent