DNA amplification

Question 1

PCR steps

Answer 1

1. initialisation: 94 C 30 secs = denaturing
2. denaturation: 94 C 30 secs = splitting to 2x singles
3. Annealing: 64 C 30 secs = primers bind
4. extension: 72 C 30 secs = synthesising complete strand
5. cycle: repeat as many times as needed

Question 2

PCR solution components

Answer 2

1. template
2. primers
3. Taq polymerase - heat resistant version of polymerase
4. dNTPs
5. Buffers and salt - for stabilisation