Gas Chromatography Deck (Exam 2) Flashcards
If gas chromatography is like a race, what is the polar stationary phase?
The track
If gas chromatography is like a race, what are the more polar components similar to?
Athletes wearing sticky shoes
If gas chromatography is like a race, what are the less polar components similar to?
Athletes wearing normal shoes
What is the stationary phase in GC composed of?
Very viscous, high-boiling liquid
What is the mobile phase in GC composed of?
Inert, non-reactive gases
Is GC the most versatile type of chromatography?
Yes
What are the 2 GC injector modes?
1) Split
2) Splitless
Where does most of the gas go in with a GC split injector?
Out of split vent
Where does most of the gas go in with a GC splitless injector?
Into the column
This is the plot of the detector’s response versus the time it takes each component to get through the instrument.
What is a chromatogram?
How is retention time useful in gas chromatography?
It can give a tentative identity to an unknown substance based on reference samples
What is the purpose of derivatization in GC?
To make compounds volatile and thermally stable when going into the gas chromatograph’s oven.
What are the 3 uses of headspace GC (HINT: headspace)?
1) Analyzing BAC
2) Arson residues
3) Hair
This GC law states, “at a constant temperature, ethanol concentration in the headspace is proportional to ethanol concentration in solution.”
What is Henry’s Law?
This is the thermal decomposition of organic matter into volatile molecules in anaerobic conditions.
What is pyrolysis?
