Chromatography Overview Deck (Exam 2)

Question 1

What are the 3 types/classes of chromatography?

Answer 1

1) Physio-chemical forces
2) Preparation technique
3) Mobile phase density

Question 2

Which type/class of chromatography do column and planar methods fall under?

Answer 2

Preparation technique

Question 3

Which type/class of chromatography do gas and liquid chromatography fall under?

Answer 3

Mobile phase density

Question 4

Why do we need chromatography?

Answer 4

Evidences such as seized drugs and biological samples are mixtures/mixed spectra, which are nearly uninterpretable

Question 5

This is the separation process based on the differential distribution of sample components between a moving and stationary phase.

Answer 5

What is chromatography?

Question 6

Chromatography requires the use of two { } phases.

Answer 6

Immiscible

Question 7

What does chromatography literally mean?

Answer 7

Color writing

Question 8

Why did Mikhail Tswett call chromatography “chromatography?”

Answer 8

Because the results of the analysis were written in color

Question 9

This is the distance between chromatograph peaks.

Answer 9

What is resolution?

Question 10

This is the width of a chromatograph peak.

Answer 10

What is efficacy?

Question 11

This is the shape of a chromatograph peak.

Answer 11

What is symmetry?

Question 12

What type of chemical interaction messes with the efficacy and symmetry of a chromatograph?

Answer 12

Dipole-dipole

Question 13

This is the amount of time a chromatograph peak elutes i.e. spends time on the column.

Answer 13

What is retention?

Question 14

Is chromatography confirmative?

Answer 14

NO

Question 15

Chromatography depends on { } distribution.

Answer 15

Differential

Question 16

What is the general principle of chromatography?

Answer 16

While one phase in held in place (stationary phase), the other moves past (mobile phase)