Fluorescence Spectroscopy (Exam 3)

Question 1

What is the working principle/mechanism behind fluorescence?

Answer 1

Light is emitted as a result of absorbing light from another source

Question 2

What are the 4 characteristics of fluorescence?

Answer 2

1) Result of light absorption
2) Occurs only during light absorption
3) Light is emitted
4) Outside light/energy source required

Question 3

What is the difference between the lifetimes of fluorescence and phosphorescence?

Answer 3

Fluorescence - longer
Phosphorescence - shorter

Question 4

How many times more sensitive is fluorescence than UV-Vis?

Answer 4

1000x

Question 5

What is the relationship between excitation, emission, and absorption in the UV-Vis region?

Answer 5

Emission usually occurs at energies that are equal to the energies of excitation

Question 6

Why is the detector placed perpendicular to the incident light?

Answer 6

To accurately see how much light has been emitted