GABA pt 3 Flashcards
1
Q
what is the IC50
A
the conc of cold diazepam required to displace 50% of the specific binding of 3H-flunitrazepam
2
Q
why is the binding assay important
A
it provides info on the affinity of the drug for thhe receptor, plus info on whether the drug exhibits binding selectivity for a particular GABAaR subtype
3
Q
what is medium throughput electrophysiology
A
requirement to determine the efficacy of either:
1) many chemical modifications of the active drug or,
2) screen large chemical libraries of compounds for efficacy
4
Q
what is the difference between phasic and tonic inhibition
A
phasic - fast current
tonic - takes a couple minutes to occur
